CSNK1G3 (NM_001031812) Human Tagged ORF Clone

CAT#: RC207965

CSNK1G3 (Myc-DDK-tagged)-Human casein kinase 1, gamma 3 (CSNK1G3), transcript variant 2

Product Images

Circular map for RC207965

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  Western blot validation of overexpression lysate (Cat# LY422199) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207965 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CSNK1G3 protein (Cat# TP307965). The protein was produced from HEK293T cells transfected with CSNK1G3 cDNA clone (Cat# RC207965) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSNK1G3
• Synonyms: CKI-gamma 3; CSNK1G3L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207965 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATAAAAAGAAAGACAAGGACAAATCAGATGATAGAATGGCACGACCTAGTGGTCGATCGGGAC
  ACAACACTCGAGGAACTGGGTCTTCATCGTCTGGAGTTTTAATGGTTGGACCTAACTTTAGAGTTGGAAA
  AAAAATTGGATGTGGCAATTTTGGAGAATTACGATTAGGGAAAAATTTATACACAAATGAATATGTGGCA
  ATTAAGTTGGAGCCCATGAAATCAAGAGCACCACAGCTACATTTGGAATACAGATTCTATGAGCAGTTAG
  GATCTGGAGATGGTATACCTCAAGTTTACTATTTCGGCCCTTGTGGTAAATACAATGCTATGGTGCTGGA
  ACTGCTGGGACCTAGTTTGGAAGACTTGTTTGACTTGTGTGACAGAACATTTTCTCTTAAAACAGTTCTC
  ATGATAGCTATACAACTGATTTCTCGCATGGAATATGTCCATTCAAAGAACTTGATATACAGAGATGTAA
  AACCTGAGAACTTCTTAATAGGACGACCAGGAAACAAAACCCAGCAAGTTATTCACATTATAGATTTTGG
  TTTGGCAAAGGAATATATTGATCCGGAGACAAAGAAACACATACCATACAGAGAACACAAGAGCCTTACA
  GGAACAGCTAGATATATGAGCATAAACACACATTTAGGAAAAGAACAAAGTAGAAGAGACGATTTAGAAG
  CTTTAGGTCATATGTTCATGTATTTTCTGAGAGGCAGTCTTCCTTGGCAAGGCTTAAAGGCTGACACATT
  AAAGGAGAGGTATCAGAAAATTGGAGATACAAAACGGGCTACACCAATAGAAGTGTTATGTGAAAATTTT
  CCAGAAATGGCAACATATCTTCGTTATGTAAGAAGGCTAGATTTTTTTGAAAAACCAGACTATGACTACT
  TAAGAAAGCTTTTTACTGACTTGTTTGATCGAAAAGGATATATGTTTGATTATGAATATGACTGGATTGG
  TAAACAGTTGCCTACTCCAGTGGGTGCAGTTCAGCAAGATCCTGCTCTGTCATCAAACAGAGAAGCACAT
  CAACACAGAGATAAGATGCAACAATCCAAAAACCAGGTTGTAAGTTCTACAAATGGAGAGTTAAACACAG
  ATGACCCCACCGCAGGACGTTCAAATGCACCCATCACAGCCCCTACTGAAGTAGAAGTGATGGATGAAAC
  CAACTGCCAGAAAGTGTTGAACATGTGGTGCTGCTGTTTTTTCAAACGAAGGAAAAGGAAAACCATACAG
  CGCCACAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207965 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MENKKKDKDKSDDRMARPSGRSGHNTRGTGSSSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVA
  IKLEPMKSRAPQLHLEYRFYEQLGSGDGIPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFSLKTVL
  MIAIQLISRMEYVHSKNLIYRDVKPENFLIGRPGNKTQQVIHIIDFGLAKEYIDPETKKHIPYREHKSLT
  GTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENF
  PEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRKGYMFDYEYDWIGKQLPTPVGAVQQDPALSSNREAH
  QHRDKMQQSKNQVVSSTNGELNTDDPTAGRSNAPITAPTEVEVMDETNCQKVLNMWCCCFFKRRKRKTIQ
  RHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031812
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031812.3, NP_001026982.1
• RefSeq Size: 4651 bp
• RefSeq ORF: 1272 bp
• Locus ID: 1456
• UniProt ID: Q9Y6M4
• Cytogenetics: 5q23.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Hedgehog signaling pathway
• MW: 48.9 kDa
• Gene Summary: This gene encodes a member of a family of serine/threonine protein kinases that phosphorylate caseins and other acidic proteins. A related protein in the African clawed frog participates in the transmission of Wnt/beta-catenin signaling. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]