HLTF (NM_003071) Human Tagged ORF Clone

CAT#: RC207920

HLTF (Myc-DDK-tagged)-Human helicase-like transcription factor (HLTF), transcript variant 1

Product Images

Circular map for RC207920

• 

  Western blot validation of overexpression lysate (Cat# LY401071) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207920 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HLTF
• Synonyms: HIP116; HIP116A; HLTF1; RNF80; SMARCA3; SNF2L3; ZBU1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207920 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCTGGATGTTCAAGAGGGATCCAGTTTGGAAGTACTTGCAGACTGTCCAGTATGGAGTTCATGGAA
  ATTTTCCACGCCTCTCATATCCAACTTTCTTTCCACGTTTTGAATTCCAAGATGTTATCCCTCCAGATGA
  CTTTCTAACTAGTGATGAAGAAGTAGATTCCGTTTTATTTGGAAGTTTGAGAGGTCATGTGGTTGGACTA
  CGCTATTACACGGGAGTAGTTAATAATAATGAAATGGTTGCATTACAACGAGATCCTAATAACCCTTATG
  ATAAGAATGCAATTAAAGTAAACAATGTGAATGGAAATCAAGTTGGCCATTTAAAGAAAGAGCTTGCAGG
  TGCTTTGGCCTATATCATGGACAACAAATTGGCACAAATTGAAGGGGTAGTTCCTTTTGGTGCAAACAAT
  GCTTTTACCATGCCTCTGCATATGACTTTTTGGGGAAAAGAAGAAAATAGAAAAGCGGTTTCAGATCAGT
  TGAAGAAACATGGATTTAAATTGGGTCCTGCACCAAAAACTTTAGGATTCAATTTGGAAAGTGGTTGGGG
  CTCTGGAAGAGCTGGACCAAGCTATAGTATGCCAGTGCATGCTGCAGTACAGATGACAACTGAACAGCTT
  AAAACAGAATTTGACAAATTGTTTGAAGATTTAAAAGAAGATGATAAAACCCATGAAATGGAACCAGCTG
  AGGCTATTGAAACACCACTGCTTCCACATCAAAAACAAGCTCTAGCTTGGATGGTGTCACGGGAAAATAG
  CAAAGAACTTCCACCATTCTGGGAACAGCGAAATGACTTATACTATAACACAATAACAAATTTTTCTGAG
  AAGGACCGACCAGAAAATGTCCATGGAGGAATTTTAGCTGATGATATGGGTTTGGGTAAAACTCTTACAG
  CCATTGCAGTAATCCTTACCAACTTCCATGATGGCAGACCTCTTCCTATTGAAAGAGTTAAAAAGAATCT
  ACTGAAGAAGGAATATAATGTTAACGATGACTCTATGAAACTTGGAGGAAACAATACCAGTGAAAAGGCA
  GATGGACTAAGCAAAGACGCATCTAGATGTAGTGAACAACCCAGTATTTCAGATATCAAGGAGAAGAGTA
  AGTTTCGCATGTCAGAATTGTCTAGCTCCCGCCCCAAAAGAAGAAAAACTGCTGTCCAGTACATAGAAAG
  CAGTGATTCAGAGGAAATTGAAACAAGTGAATTGCCGCAGAAAATGAAAGGCAAACTGAAAAATGTACAG
  TCTGAAACTAAAGGCAGGGCGAAAGGATCTTCTAAGGTTATAGAAGATGTGGCATTTGCATGTGCATTAA
  CTTCATCTGTTCCTACAACAAAAAAGAAAATGTTGAAAAAGGGAGCTTGTGCAGTGGAGGGGTCAAAGAA
  AACTGATGTTGAGGAGAGACCAAGAACAACACTGATCATCTGTCCGCTTTCTGTGTTAAGCAACTGGATT
  GACCAGTTTGGACAACATATAAAATCAGATGTACACTTGAATTTTTATGTTTATTATGGTCCTGATCGTA
  TTAGAGAACCGGCCTTACTTTCAAAACAGGATATTGTTTTGACTACGTATAATATTTTAACTCATGACTA
  TGGAACTAAAGGAGATAGTCCATTACATAGCATAAGGTGGCTAAGAGTGATCCTGGATGAAGGACATGCC
  ATACGAAATCCAAATGCTCAGCAGACAAAAGCTGTACTTGACTTAGAATCAGAAAGAAGATGGGTTTTGA
  CAGGTACTCCAATCCAGAATTCTTTAAAGGACTTGTGGTCTCTTCTTTCCTTTTTAAAACTTAAACCATT
  TATTGATAGAGAATGGTGGCATAGAACAATACAGCGTCCTGTCACAATGGGAGATGAAGGAGGACTTAGG
  CGTTTACAGTCCCTAATTAAAAATATTACACTTAGAAGAACAAAGACAAGCAAAATTAAAGGAAAACCTG
  TTTTGGAGTTACCAGAACGTAAAGTATTTATTCAGCACATTACACTTTCAGATGAAGAGAGAAAGATTTA
  TCAGTCTGTGAAAAATGAAGGCAGAGCCACTATTGGAAGGTATTTTAATGAAGGGACTGTCCTGGCACAT
  TATGCAGATGTCCTGGGTCTTTTGCTTAGACTGCGGCAAATTTGTTGCCATACTTACCTTCTTACAAATG
  CAGTGTCTTCCAATGGCCCCTCAGGAAATGATACACCTGAAGAACTGAGAAAGAAGTTAATAAGGAAGAT
  GAAGTTAATTCTGAGCTCAGGTTCAGATGAGGAATGTGCAATTTGCCTGGATTCTTTAACAGTTCCTGTG
  ATAACACATTGTGCACATGTATTTTGTAAACCCTGTATTTGCCAAGTCATTCAGAATGAGCAGCCACATG
  CTAAATGCCCTTTATGCAGAAATGATATACATGAAGATAATTTATTAGAATGTCCTCCAGAAGAATTAGC
  ACGTGACAGTGAGAAAAAGTCTGATATGGAATGGACATCCAGTTCAAAGATTAATGCGCTAATGCACGCA
  TTGACTGACTTAAGAAAGAAGAATCCCAACATAAAAAGTTTGGTTGTTTCTCAGTTTACAACATTCCTGT
  CTTTAATAGAAATACCACTTAAAGCCTCTGGATTTGTGTTTACTCGTTTGGATGGTTCCATGGCCCAAAA
  GAAAAGAGTTGAATCAATTCAGTGTTTTCAAAACACTGAAGCAGGATCTCCAACTATAATGCTTCTGTCC
  TTAAAAGCAGGTGGAGTTGGTTTGAATCTGTCTGCAGCTTCTCGAGTGTTTTTAATGGATCCAGCCTGGA
  ATCCTGCTGCTGAAGATCAGTGCTTTGACAGATGCCATAGACTTGGTCAGAAGCAAGAAGTTATCATCAC
  AAAATTCATTGTAAAGGACTCTGTTGAAGAAAATATGCTGAAAATACAAAACAAAAAGAGAGAACTTGCA
  GCAGGAGCCTTTGGAACTAAAAAACCAAATGCTGACGAAATGAAACAAGCCAAAATTAATGAAATCAGAA
  CATTAATTGACTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207920 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSWMFKRDPVWKYLQTVQYGVHGNFPRLSYPTFFPRFEFQDVIPPDDFLTSDEEVDSVLFGSLRGHVVGL
  RYYTGVVNNNEMVALQRDPNNPYDKNAIKVNNVNGNQVGHLKKELAGALAYIMDNKLAQIEGVVPFGANN
  AFTMPLHMTFWGKEENRKAVSDQLKKHGFKLGPAPKTLGFNLESGWGSGRAGPSYSMPVHAAVQMTTEQL
  KTEFDKLFEDLKEDDKTHEMEPAEAIETPLLPHQKQALAWMVSRENSKELPPFWEQRNDLYYNTITNFSE
  KDRPENVHGGILADDMGLGKTLTAIAVILTNFHDGRPLPIERVKKNLLKKEYNVNDDSMKLGGNNTSEKA
  DGLSKDASRCSEQPSISDIKEKSKFRMSELSSSRPKRRKTAVQYIESSDSEEIETSELPQKMKGKLKNVQ
  SETKGRAKGSSKVIEDVAFACALTSSVPTTKKKMLKKGACAVEGSKKTDVEERPRTTLIICPLSVLSNWI
  DQFGQHIKSDVHLNFYVYYGPDRIREPALLSKQDIVLTTYNILTHDYGTKGDSPLHSIRWLRVILDEGHA
  IRNPNAQQTKAVLDLESERRWVLTGTPIQNSLKDLWSLLSFLKLKPFIDREWWHRTIQRPVTMGDEGGLR
  RLQSLIKNITLRRTKTSKIKGKPVLELPERKVFIQHITLSDEERKIYQSVKNEGRATIGRYFNEGTVLAH
  YADVLGLLLRLRQICCHTYLLTNAVSSNGPSGNDTPEELRKKLIRKMKLILSSGSDEECAICLDSLTVPV
  ITHCAHVFCKPCICQVIQNEQPHAKCPLCRNDIHEDNLLECPPEELARDSEKKSDMEWTSSSKINALMHA
  LTDLRKKNPNIKSLVVSQFTTFLSLIEIPLKASGFVFTRLDGSMAQKKRVESIQCFQNTEAGSPTIMLLS
  LKAGGVGLNLSAASRVFLMDPAWNPAAEDQCFDRCHRLGQKQEVIITKFIVKDSVEENMLKIQNKKRELA
  AGAFGTKKPNADEMKQAKINEIRTLIDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003071
• ORF Size: 3024 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003071.4
• RefSeq Size: 5351 bp
• RefSeq ORF: 3030 bp
• Locus ID: 6596
• UniProt ID: Q14527
• Cytogenetics: 3q24
• Domains: SNF2_N, DEAD, helicase_C, RING
• Protein Families: Druggable Genome, Transcription Factors
• MW: 113.9 kDa
• Gene Summary: This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]