GAS 6 (GAS6) (NM_000820) Human Tagged ORF Clone

CAT#: RC207916

GAS6 (Myc-DDK-tagged)-Human growth arrest-specific 6 (GAS6), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY424508) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207916 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GAS6
• Synonyms: AXLLG; AXSF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207916 representing NM_000820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTTCGCTCTCGCCCGGGCCCGCCGCCCTGCGCCGCGCGCCGCAGCTGCTGCTGCTGCTGCTGG
  CCGCGGAGTGCGCGCTTGCCGCGCTGTTGCCGGCGCGCGAGGCCACGCAGTTCCTGCGGCCCAGGCAGCG
  CCGCGCCTTTCAGGTCTTCGAGGAGGCCAAGCAGGGCCACCTGGAGAGGGAGTGCGTGGAGGAGCTGTGC
  AGCCGCGAGGAGGCGCGGGAGGTGTTCGAGAACGACCCCGAGACGGATTATTTTTACCCAAGATACTTAG
  ACTGCATCAACAAGTATGGGTCTCCGTACACCAAAAACTCAGGCTTCGCCACCTGCGTGCAAAACCTGCC
  TGACCAGTGCACGCCCAACCCCTGCGATAGGAAGGGGACCCAAGCCTGCCAGGACCTCATGGGCAACTTC
  TTCTGCCTGTGTAAAGCTGGCTGGGGGGGCCGGCTCTGCGACAAAGATGTCAACGAATGCAGCCAGGAGA
  ACGGGGGCTGCCTCCAGATCTGCCACAACAAGCCGGGTAGCTTCCACTGTTCCTGCCACAGCGGCTTCGA
  GCTCTCCTCTGATGGCAGGACCTGCCAAGACATAGACGAGTGCGCAGACTCGGAGGCCTGCGGGGAGGCG
  CGCTGCAAGAACCTGCCCGGCTCCTACTCCTGCCTCTGTGACGAGGGCTTTGCGTACAGCTCCCAGGAGA
  AGGCTTGCCGAGATGTGGACGAGTGTCTGCAGGGCCGCTGTGAGCAGGTCTGCGTGAACTCCCCAGGGAG
  CTACACCTGCCACTGTGACGGGCGTGGGGGCCTCAAGCTGTCCCAGGACATGGACACCTGTGAGGACATC
  TTGCCGTGCGTGCCCTTCAGCGTGGCCAAGAGTGTGAAGTCCTTGTACCTGGGCCGGATGTTCAGTGGGA
  CCCCCGTGATCCGACTGCGCTTCAAGAGGCTGCAGCCCACCAGGCTGGTAGCTGAGTTTGACTTCCGGAC
  CTTTGACCCCGAGGGCATCCTCCTCTTTGCCGGAGGCCACCAGGACAGCACCTGGATCGTGCTGGCCCTG
  AGAGCCGGCCGGCTGGAGCTGCAGCTGCGCTACAACGGTGTCGGCCGTGTCACCAGCAGCGGCCCGGTCA
  TCAACCATGGCATGTGGCAGACAATCTCTGTTGAGGAGCTGGCGCGGAATCTGGTCATCAAGGTCAACAG
  GGATGCTGTCATGAAAATCGCGGTGGCCGGGGACTTGTTCCAACCGGAGCGAGGACTGTATCATCTGAAC
  CTGACCGTGGGAGGTATTCCCTTCCATGAGAAGGACCTCGTGCAGCCTATAAACCCTCGTCTGGATGGCT
  GCATGAGGAGCTGGAACTGGCTGAACGGAGAAGACACCACCATCCAGGAAACGGTGAAAGTGAACACGAG
  GATGCAGTGCTTCTCGGTGACGGAGAGAGGCTCTTTCTACCCCGGGAGCGGCTTCGCCTTCTACAGCCTG
  GACTACATGCGGACCCCTCTGGACGTCGGGACTGAATCAACCTGGGAAGTAGAAGTCGTGGCTCACATCC
  GCCCAGCCGCAGACACAGGCGTGCTGTTTGCGCTCTGGGCCCCCGACCTCCGTGCCGTGCCTCTCTCTGT
  GGCACTGGTAGACTATCACTCCACGAAGAAACTCAAGAAGCAGCTGGTGGTCCTGGCCGTGGAGCATACG
  GCCTTGGCCCTAATGGAGATCAAGGTCTGCGACGGCCAAGAGCACGTGGTCACCGTCTCGCTGAGGGACG
  GTGAGGCCACCCTGGAGGTGGACGGCACCAGGGGCCAGAGCGAGGTGAGCGCCGCGCAGCTGCAGGAGAG
  GCTGGCCGTGCTCGAGAGGCACCTGCGGAGCCCCGTGCTCACCTTTGCTGGCGGCCTGCCAGATGTGCCG
  GTGACTTCAGCGCCAGTCACCGCGTTCTACCGCGGCTGCATGACACTGGAGGTCAACCGGAGGCTGCTGG
  ACCTGGACGAGGCGGCGTACAAGCACAGCGACATCACGGCCCACTCCTGCCCCCCCGTGGAGCCCGCCGC
  AGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207916 representing NM_000820
  Red=Cloning site Green=Tags(s)
  
  MAPSLSPGPAALRRAPQLLLLLLAAECALAALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELC
  SREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNF
  FCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEA
  RCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDI
  LPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLAL
  RAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLN
  LTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSL
  DYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHT
  ALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVP
  VTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000820
• ORF Size: 2034 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000820.4
• RefSeq Size: 2461 bp
• RefSeq ORF: 2037 bp
• Locus ID: 2621
• UniProt ID: Q14393
• Cytogenetics: 13q34
• Domains: GLA, LamG, EGF_CA, EGF, EGF
• Protein Families: Druggable Genome, Secreted Protein
• MW: 74.93 kDa
• Gene Summary: This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]