GAS 6 (GAS6) (NM_000820) Human Tagged ORF Clone

CAT#: RC207916

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GAS6 (Myc-DDK-tagged)-Human growth arrest-specific 6 (GAS6), transcript variant 1



  "NM_000820" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 691.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GAS6
Synonyms AXLLG; AXSF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207916 representing NM_000820
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCTTCGCTCTCGCCCGGGCCCGCCGCCCTGCGCCGCGCGCCGCAGCTGCTGCTGCTGCTGCTGG
CCGCGGAGTGCGCGCTTGCCGCGCTGTTGCCGGCGCGCGAGGCCACGCAGTTCCTGCGGCCCAGGCAGCG
CCGCGCCTTTCAGGTCTTCGAGGAGGCCAAGCAGGGCCACCTGGAGAGGGAGTGCGTGGAGGAGCTGTGC
AGCCGCGAGGAGGCGCGGGAGGTGTTCGAGAACGACCCCGAGACGGATTATTTTTACCCAAGATACTTAG
ACTGCATCAACAAGTATGGGTCTCCGTACACCAAAAACTCAGGCTTCGCCACCTGCGTGCAAAACCTGCC
TGACCAGTGCACGCCCAACCCCTGCGATAGGAAGGGGACCCAAGCCTGCCAGGACCTCATGGGCAACTTC
TTCTGCCTGTGTAAAGCTGGCTGGGGGGGCCGGCTCTGCGACAAAGATGTCAACGAATGCAGCCAGGAGA
ACGGGGGCTGCCTCCAGATCTGCCACAACAAGCCGGGTAGCTTCCACTGTTCCTGCCACAGCGGCTTCGA
GCTCTCCTCTGATGGCAGGACCTGCCAAGACATAGACGAGTGCGCAGACTCGGAGGCCTGCGGGGAGGCG
CGCTGCAAGAACCTGCCCGGCTCCTACTCCTGCCTCTGTGACGAGGGCTTTGCGTACAGCTCCCAGGAGA
AGGCTTGCCGAGATGTGGACGAGTGTCTGCAGGGCCGCTGTGAGCAGGTCTGCGTGAACTCCCCAGGGAG
CTACACCTGCCACTGTGACGGGCGTGGGGGCCTCAAGCTGTCCCAGGACATGGACACCTGTGAGGACATC
TTGCCGTGCGTGCCCTTCAGCGTGGCCAAGAGTGTGAAGTCCTTGTACCTGGGCCGGATGTTCAGTGGGA
CCCCCGTGATCCGACTGCGCTTCAAGAGGCTGCAGCCCACCAGGCTGGTAGCTGAGTTTGACTTCCGGAC
CTTTGACCCCGAGGGCATCCTCCTCTTTGCCGGAGGCCACCAGGACAGCACCTGGATCGTGCTGGCCCTG
AGAGCCGGCCGGCTGGAGCTGCAGCTGCGCTACAACGGTGTCGGCCGTGTCACCAGCAGCGGCCCGGTCA
TCAACCATGGCATGTGGCAGACAATCTCTGTTGAGGAGCTGGCGCGGAATCTGGTCATCAAGGTCAACAG
GGATGCTGTCATGAAAATCGCGGTGGCCGGGGACTTGTTCCAACCGGAGCGAGGACTGTATCATCTGAAC
CTGACCGTGGGAGGTATTCCCTTCCATGAGAAGGACCTCGTGCAGCCTATAAACCCTCGTCTGGATGGCT
GCATGAGGAGCTGGAACTGGCTGAACGGAGAAGACACCACCATCCAGGAAACGGTGAAAGTGAACACGAG
GATGCAGTGCTTCTCGGTGACGGAGAGAGGCTCTTTCTACCCCGGGAGCGGCTTCGCCTTCTACAGCCTG
GACTACATGCGGACCCCTCTGGACGTCGGGACTGAATCAACCTGGGAAGTAGAAGTCGTGGCTCACATCC
GCCCAGCCGCAGACACAGGCGTGCTGTTTGCGCTCTGGGCCCCCGACCTCCGTGCCGTGCCTCTCTCTGT
GGCACTGGTAGACTATCACTCCACGAAGAAACTCAAGAAGCAGCTGGTGGTCCTGGCCGTGGAGCATACG
GCCTTGGCCCTAATGGAGATCAAGGTCTGCGACGGCCAAGAGCACGTGGTCACCGTCTCGCTGAGGGACG
GTGAGGCCACCCTGGAGGTGGACGGCACCAGGGGCCAGAGCGAGGTGAGCGCCGCGCAGCTGCAGGAGAG
GCTGGCCGTGCTCGAGAGGCACCTGCGGAGCCCCGTGCTCACCTTTGCTGGCGGCCTGCCAGATGTGCCG
GTGACTTCAGCGCCAGTCACCGCGTTCTACCGCGGCTGCATGACACTGGAGGTCAACCGGAGGCTGCTGG
ACCTGGACGAGGCGGCGTACAAGCACAGCGACATCACGGCCCACTCCTGCCCCCCCGTGGAGCCCGCCGC
AGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207916 representing NM_000820
Red=Cloning site Green=Tags(s)

MAPSLSPGPAALRRAPQLLLLLLAAECALAALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELC
SREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNF
FCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEA
RCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDI
LPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLAL
RAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLN
LTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSL
DYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHT
ALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVP
VTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000820
ORF Size 2034 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000820.4
RefSeq Size 2461 bp
RefSeq ORF 2037 bp
Locus ID 2621
UniProt ID Q14393
Cytogenetics 13q34
Domains GLA, LamG, EGF_CA, EGF, EGF
Protein Families Druggable Genome, Secreted Protein
MW 74.93 kDa
Gene Summary This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]

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