JARID2 (NM_004973) Human Tagged Lenti ORF Clone
- LentiORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | JARID2 |
| Synonyms | JMJ |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_004973 |
| ORF Size | 3738 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_004973.2 |
| RefSeq Size | 5756 bp |
| RefSeq ORF | 3741 bp |
| Locus ID | 3720 |
| UniProt ID | Q92833 |
| Cytogenetics | 6p22.3 |
| Domains | ARID, JmjC, JmjN, zf-C5HC2 |
| Protein Families | Transcription Factors |
| MW | 138.6 kDa |
| Summary | This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. provided by RefSeq, May 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_004973" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207885 | JARID2 (Myc-DDK-tagged)-Human jumonji, AT rich interactive domain 2 (JARID2) | 10 ug |
$589.00
$1,085.00
|
|
| RC207885L1 | Lenti-ORF clone of JARID2 (Myc-DDK-tagged)-Human jumonji, AT rich interactive domain 2 (JARID2) | 10 ug |
$1,385.00
|
|
| RC207885L2 | Lenti-ORF clone of JARID2 (mGFP-tagged)-Human jumonji, AT rich interactive domain 2 (JARID2) | 10 ug |
$1,385.00
|
|
| RC207885L4 | Lenti-ORF clone of JARID2 (mGFP-tagged)-Human jumonji, AT rich interactive domain 2 (JARID2) | 10 ug |
$1,385.00
|
|
| RG207885 | JARID2 (tGFP-tagged) - Human jumonji, AT rich interactive domain 2 (JARID2) | 10 ug |
$789.00
$1,285.00
|
|
| SC309940 | JARID2 (untagged)-Human jumonji, AT rich interactive domain 2 (JARID2) | 10 ug |
$1,194.00
|
|
Plasmid Map for RC207885L3
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Cloning scheme for RC207885L3
Circular map for RC207885L3
Citations
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