RPAP2 (NM_024813) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207882L4
Lenti ORF clone of Human RNA polymerase II associated protein 2 (RPAP2), mGFP tagged
  $870.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPAP2
Synonyms C1orf82; Rtr1
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207882).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024813
ORF Size 1836 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024813.1
RefSeq Size 3112 bp
RefSeq ORF 1839 bp
Locus ID 79871
UniProt ID Q8IXW5
Cytogenetics 1p22.1
Domains DUF408
MW 69.5 kDa
Summary Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024813" in other vectors (4)
SKU Description Size Price
RC207882 RPAP2 (Myc-DDK-tagged)-Human RNA polymerase II associated protein 2 (RPAP2) 10 ug
$570.00
RC207882L3 Lenti ORF clone of Human RNA polymerase II associated protein 2 (RPAP2), Myc-DDK-tagged 10 ug
$870.00
RG207882 RPAP2 (tGFP-tagged) - Human RNA polymerase II associated protein 2 (RPAP2) 10 ug
$489.00 $770.00
SC111990 RPAP2 (untagged)-Human RNA polymerase II associated protein 2 (RPAP2) 10 ug
$571.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.