GRHL3 (NM_198174) Human Tagged ORF Clone

CAT#: RC207857

GRHL3 (Myc-DDK-tagged)-Human grainyhead-like 3 (Drosophila) (GRHL3), transcript variant 3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GRHL3 (Cat# RC207857, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHL3 (Cat# TA810684)(1:2000). Positive lysates LY404981 (100ug) and LC404981 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY404981) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207857 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GRHL3 protein (Cat# TP307857). The protein was produced from HEK293T cells transfected with GRHL3 cDNA clone (Cat# RC207857) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRHL3
• Synonyms: SOM; TFCP2L4; VWS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207857 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGTCAATGGAGATGATGACAGTGTTGCGGCCTTGAGCTTCCTCTATGATTACTACATGGGTCCCA
  AGGAGAAGCGGATATTGTCCTCCAGCACTGGGGGCAGGAATGACCAAGGAAAGAGGTACTACCATGGCAT
  GGAATATGAGACGGACCTCACTCCCCTTGAAAGCCCCACACACCTCATGAAATTCCTGACAGAGAACGTG
  TCTGGAACCCCAGAGTACCCAGATTTGCTCAAGAAGAATAACCTGATGAGCTTGGAGGGGGCCTTGCCCA
  CCCCTGGCAAGGCAGCTCCCCTCCCTGCAGGCCCCAGCAAGCTGGAGGCCGGCTCTGTGGACAGCTACCT
  GTTACCCACCACTGATATGTATGATAATGGCTCCCTCAACTCCTTGTTTGAGAGCATTCATGGGGTGCCG
  CCCACACAGCGCTGGCAGCCAGACAGCACCTTCAAAGATGACCCACAGGAGTCGATGCTCTTCCCAGATA
  TCCTGAAAACCTCCCCGGAACCCCCATGTCCAGAGGACTACCCCAGCCTCAAAAGTGACTTTGAATACAC
  CCTGGGCTCCCCCAAAGCCATCCACATCAAGTCAGGCGAGTCACCCATGGCCTACCTCAACAAAGGCCAG
  TTCTACCCCGTCACCCTGCGGACCCCAGCAGGTGGCAAAGGCCTTGCCTTGTCCTCCAACAAAGTCAAGA
  GTGTGGTGATGGTTGTCTTCGACAATGAGAAGGTCCCAGTAGAGCAGCTGCGCTTCTGGAAGCACTGGCA
  TTCCCGGCAACCCACTGCCAAGCAGCGGGTCATTGACGTGGCTGACTGCAAAGAAAACTTCAACACTGTG
  GAGCACATTGAGGAGGTGGCCTATAATGCACTGTCCTTTGTGTGGAACGTGAATGAAGAGGCCAAGGTGT
  TCATCGGCGTAAACTGTCTGAGCACAGACTTTTCCTCACAAAAGGGGGTGAAGGGTGTCCCCCTGAACCT
  GCAGATTGACACCTATGACTGTGGCTTGGGCACTGAGCGCCTGGTACACCGTGCTGTCTGCCAGATCAAG
  ATCTTCTGTGACAAGGGAGCTGAGAGGAAGATGCGCGATGACGAGCGGAAGCAGTTCCGGAGGAAGGTCA
  AGTGCCCTGACTCCAGCAACAGTGGCGTCAAGGGCTGCCTGCTGTCGGGCTTCAGGGGCAATGAGACGAC
  CTACCTTCGGCCAGAGACTGACCTGGAGACGCCACCCGTGCTGTTCATCCCCAATGTGCACTTCTCCAGC
  CTGCAGCGCTCTGGAGGGGCAGCCCCCTCGGCAGGACCCAGCAGCTCCAACAGGCTGCCTCTGAAGCGTA
  CCTGCTCGCCCTTCACTGAGGAGTTTGAGCCTCTGCCCTCCAAGCAGGCCAAGGAAGGCGACCTTCAGAG
  AGTTCTGCTGTATGTGCGGAGGGAGACTGAGGAGGTGTTTGACGCGCTCATGTTGAAGACCCCAGACCTG
  AAGGGGCTGAGGAATGCGATCTCTGAGAAGTATGGGTTCCCTGAAGAGAACATTTACAAAGTCTACAAGA
  AATGCAAGCGAGGAATCTTAGTCAACATGGACAACAACATCATTCAGCATTACAGCAACCACGTCGCCTT
  CCTGCTGGACATGGGGGAGCTGGACGGCAAAATTCAGATCATCCTTAAGGAGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207857 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRVNGDDDSVAALSFLYDYYMGPKEKRILSSSTGGRNDQGKRYYHGMEYETDLTPLESPTHLMKFLTENV
  SGTPEYPDLLKKNNLMSLEGALPTPGKAAPLPAGPSKLEAGSVDSYLLPTTDMYDNGSLNSLFESIHGVP
  PTQRWQPDSTFKDDPQESMLFPDILKTSPEPPCPEDYPSLKSDFEYTLGSPKAIHIKSGESPMAYLNKGQ
  FYPVTLRTPAGGKGLALSSNKVKSVVMVVFDNEKVPVEQLRFWKHWHSRQPTAKQRVIDVADCKENFNTV
  EHIEEVAYNALSFVWNVNEEAKVFIGVNCLSTDFSSQKGVKGVPLNLQIDTYDCGLGTERLVHRAVCQIK
  IFCDKGAERKMRDDERKQFRRKVKCPDSSNSGVKGCLLSGFRGNETTYLRPETDLETPPVLFIPNVHFSS
  LQRSGGAAPSAGPSSSNRLPLKRTCSPFTEEFEPLPSKQAKEGDLQRVLLYVRRETEEVFDALMLKTPDL
  KGLRNAISEKYGFPEENIYKVYKKCKRGILVNMDNNIIQHYSNHVAFLLDMGELDGKIQIILKEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198174
• ORF Size: 1668 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 2232 bp
• RefSeq ORF: 1881 bp
• Locus ID: 57822
• UniProt ID: Q8TE85
• Cytogenetics: 1p36.11
• MW: 62.2 kDa
• Gene Summary: This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Aug 2010]