PIGO (NM_152850) Human Tagged Lenti ORF Clone
- LentiORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PIGO |
| Synonyms | HPMRS2 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_152850 |
| ORF Size | 2016 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_152850.3, NP_690577.2 |
| RefSeq Size | 2482 bp |
| RefSeq ORF | 2019 bp |
| Locus ID | 84720 |
| UniProt ID | Q8TEQ8 |
| Cytogenetics | 9p13.3 |
| Protein Families | Transmembrane |
| Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
| MW | 74 kDa |
| Summary | This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. provided by RefSeq, Jan 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_152850" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207841 | PIGO (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 | 10 ug |
$677.00
|
|
| RC207841L3 | Lenti-ORF clone of PIGO (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 | 10 ug |
$977.00
|
|
| RG207841 | PIGO (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 | 10 ug |
$877.00
|
|
| SC110703 | PIGO (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 | 10 ug |
$616.00
|
|
| SC324354 | PIGO (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 | 10 ug |
$616.00
|
|
Plasmid Map for RC207841L4
Download sequence for
Cloning scheme for RC207841L4
Circular map for RC207841L4
Citations
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