PIGO (NM_152850) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207841L4
Lenti-ORF clone of PIGO (mGFP-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2
  $977.00
4 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PIGO
Synonyms HPMRS2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207841).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152850
ORF Size 2016 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152850.3, NP_690577.2
RefSeq Size 2482 bp
RefSeq ORF 2019 bp
Locus ID 84720
UniProt ID Q8TEQ8
Cytogenetics 9p13.3
Protein Families Transmembrane
Protein Pathways Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
MW 74 kDa
Summary This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many blood cells and serves to anchor proteins to the cell surface. This protein is involved in the transfer of ethanolaminephosphate (EtNP) to the third mannose in GPI. At least three alternatively spliced transcripts encoding two distinct isoforms have been found for this gene. provided by RefSeq, Jan 2011
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Documents
Resources
"NM_152850" in other vectors (5)
SKU Description Size Price
RC207841 PIGO (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 10 ug
$677.00
RC207841L3 Lenti-ORF clone of PIGO (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 10 ug
$977.00
RG207841 PIGO (tGFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 10 ug
$877.00
SC110703 PIGO (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 10 ug
$616.00
SC324354 PIGO (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class O (PIGO), transcript variant 2 10 ug
$616.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.