C13orf31 (LACC1) (NM_153218) Human Tagged ORF Clone

CAT#: RC207834

LACC1 (Myc-DDK-tagged)-Human chromosome 13 open reading frame 31 (C13orf31), transcript variant 2

Product Images

Circular map for RC207834

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  Western blot validation of overexpression lysate (Cat# LY426942) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225705 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LACC1 protein (Cat# TP307834). The protein was produced from HEK293T cells transfected with LACC1 cDNA clone (Cat# RC207834) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C13orf31
• Synonyms: C13orf31; FAMIN; JUVAR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207834 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAGCTGTTTTGATTGATCTTTTTGGTTTGAAATTGAACTCTCAAAAAAACTGCCATCAGACAT
  TACTGAAGACTTTGAATGCTGTCCAATACCACCATGCTGCCAAGGCCAAGTTTCTCTGTATAATGTGTTG
  CAGTAACATCAGCTATGAAAGGGATGGAGAACAAGATAATTGTGAAATAGAAACAAGCAATGGATTATCA
  GCTCTCTTGGAAGAATTTGAGATTGTTAGCTGTCCCAGCATGGCTGCCACTTTGTATACCATTAAACAGA
  AAATTGATGAAAAAAATCTGAGCAGCATTAAGGTAATTGTACCCAGGCACAGGAAGACATTAATGAAAGC
  TTTTATTGATCAACTCTTCACAGATGTTTACAATTTTGAATTTGAAGATTTGCAAGTGACTTTTAGGGGA
  GGGCTTTTTAAACAGTCCATTGAAATAAACGTAATCACAGCTCAAGAACTAAGAGGAATTCAGAATGAAA
  TAGAAACATTTTTGAGAAGTCTGCCAGCACTGAGAGGAAAATTAACTATTATCACTTCTTCTTTGATCCC
  AGATATTTTCATACATGGATTTACTACAAGAACAGGTGGGATATCTTATATACCAACTCTTAGCTCATTC
  AATCTCTTCAGTAGTTCCAAACGGAGAGATCCCAAGGTAGTGGTTCAAGAAAATCTGCGTAGGTTGGCGA
  ATGCTGCAGGATTTAATGTGGAGAAATTTTACCGAATAAAGACTCATCATTCCAATGACATCTGGATTAT
  GGGAAGAAAGGAGCCTGACTCTTATGATGGAATAACCACAAATCAGAGAGGAGTCACAATAGCAGCTCTT
  GGTGCAGACTGTATACCGATAGTTTTTGCAGATCCAGTCAAAAAAGCATGTGGGGTTGCTCACGCTGGTT
  GGAAAGGTACTTTGTTGGGTGTTGCTATGGCTACAGTGAATGCTATGATAGCAGAATATGGCTGCAGTTT
  GGAAGACATTGTTGTTGTACTTGGACCTTCAGTAGGACCTTGCTGTTTTACTCTTCCAAGGGAATCAGCA
  GAGGCATTTCATAATCTTCATCCTGCATGTGTACAACTATTTGATTCACCAAATCCCTGTATCGACATCC
  GTAAAGCCACAAGGATTCTTCTAGAACAGGGAGGAATTCTTCCACAGAATATTCAGGACCAGAACCAAGA
  TCTCAACCTCTGTACATCTTGCCATCCTGACAAGTTTTTCTCCCATGTCCGAGATGGCCTTAATTTTGGT
  ACACAGATTGGCTTCATATCAATTAAAGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207834 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEAVLIDLFGLKLNSQKNCHQTLLKTLNAVQYHHAAKAKFLCIMCCSNISYERDGEQDNCEIETSNGLS
  ALLEEFEIVSCPSMAATLYTIKQKIDEKNLSSIKVIVPRHRKTLMKAFIDQLFTDVYNFEFEDLQVTFRG
  GLFKQSIEINVITAQELRGIQNEIETFLRSLPALRGKLTIITSSLIPDIFIHGFTTRTGGISYIPTLSSF
  NLFSSSKRRDPKVVVQENLRRLANAAGFNVEKFYRIKTHHSNDIWIMGRKEPDSYDGITTNQRGVTIAAL
  GADCIPIVFADPVKKACGVAHAGWKGTLLGVAMATVNAMIAEYGCSLEDIVVVLGPSVGPCCFTLPRESA
  EAFHNLHPACVQLFDSPNPCIDIRKATRILLEQGGILPQNIQDQNQDLNLCTSCHPDKFFSHVRDGLNFG
  TQIGFISIKE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153218
• ORF Size: 1290 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153218.4
• RefSeq Size: 4124 bp
• RefSeq ORF: 1293 bp
• Locus ID: 144811
• UniProt ID: Q8IV20
• Cytogenetics: 13q14.11
• Domains: DUF152
• MW: 47.8 kDa
• Gene Summary: This gene encodes an oxidoreductase that promotes fatty-acid oxidation, with concomitant inflammasome activation, mitochondrial and NADPH-oxidase-dependent reactive oxygen species production, and bactericidal activity of macrophages. The encoded protein forms a complex with fatty acid synthase on peroxisomes and is thought to be modulated by peroxisome proliferator-activated receptor signaling events. Naturally occurring mutations in this gene are associated with inflammatory bowel disease, Behcet's disease, leprosy, ulcerative colitis, early-onset Crohn's disease, and systemic juvenile idiopathic arthritis. [provided by RefSeq, Apr 2017]