NSUN6 (NM_182543) Human Tagged ORF Clone

CAT#: RC207817

NSUN6 (Myc-DDK-tagged)-Human NOP2/Sun domain family, member 6 (NSUN6)

Product Images

Circular map for RC207817

• 

  Western blot validation of overexpression lysate (Cat# LY405497) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207817 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified NSUN6 protein (Cat# TP307817). The protein was produced from HEK293T cells transfected with NSUN6 cDNA clone (Cat# RC207817) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NSUN6
• Synonyms: 4933414E04Rik; ARL5B-AS1; NOPD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207817 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATTTTCCCTAAGATATCTTTGAGACCTGAGGTTGAAAACTATCTTAAGGAAGGCTTTATGAATA
  AGGAGATTGTGACTGCTTTAGGTAAACAAGAAGCAGAAAGGAAGTTTGAAACTTTGTTAAAGCACCTGTC
  ACATCCTCCATCATTTACAACTGTCAGAGTGAATACACATTTAGCCTCAGTACAACATGTGAAAAATCTG
  TTACTTGATGAACTTCAGAAGCAGTTTAATGGATTAAGTGTTCCTATTCTTCAACATCCAGACCTTCAAG
  ATGTGTTACTTATTCCTGTTATTGGACCCAGAAAGAATATTAAAAAACAACAGTGTGAAGCCATTGTTGG
  AGCCCAGTGTGGCAATGCAGTTTTAAGAGGAGCCCATGTCTATGCCCCAGGAATTGTGTCAGCATCACAA
  TTTATGAAAGCTGGAGATGTTATTTCTGTATACTCTGATATTAAAGGAAAATGTAAGAAAGGAGCCAAAG
  AATTTGATGGAACAAAAGTATTTCTTGGAAATGGGATTTCTGAACTAAGCCGCAAAGAAATCTTCAGTGG
  ATTACCTGAACTGAAAGGCATGGGCATAAGAATGACAGAACCAGTATATCTCAGCCCTTCATTTGACAGT
  GTACTGCCCCGTTACTTATTTTTACAAAATTTGCCATCTGCCTTAGTAAGTCATGTACTAAATCCTCAAC
  CTGGAGAGAAGATTCTAGACTTGTGTGCAGCACCTGGAGGGAAAACAACACACATTGCAGCACTAATGCA
  TGATCAGGGAGAAGTTATAGCACTGGATAAAATCTTCAACAAAGTAGAAAAAATCAAACAGAATGCCTTA
  TTGTTAGGGCTGAATTCCATCAGGGCATTTTGTTTTGATGGAACAAAGGCGGTTAAACTTGATATGGTGG
  AGGACACAGAAGGAGAACCTCCATTTCTACCAGAATCCTTTGACCGAATTCTTCTGGATGCACCCTGTAG
  TGGAATGGGACAGAGACCAAACATGGCCTGTACTTGGTCTGTGAAGGAAGTGGCATCATATCAGCCATTA
  CAGCGAAAACTCTTCACTGCAGCGGTTCAGCTGCTGAAGCCAGAGGGTGTGCTGGTTTATAGCACGTGCA
  CTATAACACTGGCCGAAAATGAAGAACAGGTTGCCTGGGCCCTGACAAAATTTCCTTGCCTTCAGCTTCA
  GCCCCAGGAACCGCAGATTGGAGGAGAAGGAATGAGGGGAGCTGGGCTCTCATGTGAACAGTTGAAACAG
  CTGCAGCGATTTGATCCATCGGCTGTGCCATTACCGGACACTGACATGGACTCTCTTAGAGAGGCCAGAA
  GAGAAGACATGTTGCGTCTGGCTAATAAGGACTCTATAGGTTTTTTTATTGCAAAATTTGTAAAATGCAA
  AAGCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207817 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSIFPKISLRPEVENYLKEGFMNKEIVTALGKQEAERKFETLLKHLSHPPSFTTVRVNTHLASVQHVKNL
  LLDELQKQFNGLSVPILQHPDLQDVLLIPVIGPRKNIKKQQCEAIVGAQCGNAVLRGAHVYAPGIVSASQ
  FMKAGDVISVYSDIKGKCKKGAKEFDGTKVFLGNGISELSRKEIFSGLPELKGMGIRMTEPVYLSPSFDS
  VLPRYLFLQNLPSALVSHVLNPQPGEKILDLCAAPGGKTTHIAALMHDQGEVIALDKIFNKVEKIKQNAL
  LLGLNSIRAFCFDGTKAVKLDMVEDTEGEPPFLPESFDRILLDAPCSGMGQRPNMACTWSVKEVASYQPL
  QRKLFTAAVQLLKPEGVLVYSTCTITLAENEEQVAWALTKFPCLQLQPQEPQIGGEGMRGAGLSCEQLKQ
  LQRFDPSAVPLPDTDMDSLREARREDMLRLANKDSIGFFIAKFVKCKST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182543
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182543.5
• RefSeq Size: 2570 bp
• RefSeq ORF: 1410 bp
• Locus ID: 221078
• UniProt ID: Q8TEA1
• Cytogenetics: 10p12.31
• Protein Families: Druggable Genome
• MW: 51.8 kDa
• Gene Summary: May have S-adenosyl-L-methionine-dependent methyl-transferase activity.[UniProtKB/Swiss-Prot Function]