PRKAR1B (NM_002735) Human Tagged ORF Clone

CAT#: RC207809

PRKAR1B (Myc-DDK-tagged)-Human protein kinase, cAMP-dependent, regulatory, type I, beta (PRKAR1B), transcript variant 2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PRKAR1B (Cat# RC207809, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1B (Cat# TA502750). Positive lysates LY400964 (100ug) and LC400964 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY431891) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC228863 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PRKAR1B protein (Cat# TP307809). The protein was produced from HEK293T cells transfected with PRKAR1B cDNA clone (Cat# RC207809) using MegaTran 2.0 (Cat# TT210002).

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  Anti-PRKAR1B mouse monoclonal antibody (TA502750) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1B (RC207809 ).

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  HEK293T cells transfected with either RC207809 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKAR1B antibody (TA502750), and then analyzed by flow cytometry.

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Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRKAR1B
• Synonyms: PRKAR1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207809 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCCCGCCCGCCTGCCCCTCGGAGGAGGACGAGAGCCTGAAGGGCTGTGAGCTGTACGTGCAGC
  TGCACGGGATCCAGCAGGTCCTCAAAGACTGTATCGTCCACCTCTGCATCTCCAAGCCCGAACGCCCCAT
  GAAGTTCCTCCGGGAGCACTTCGAGAAGCTGGAGAAGGAAGAAAACAGGCAGATTTTGGCGCGGCAAAAG
  TCAAACTCACAGTCGGACTCCCATGATGAGGAGGTGTCGCCCACCCCCCCGAACCCTGTGGTGAAGGCCC
  GCCGCCGGCGAGGAGGCGTGAGTGCCGAGGTGTACACCGAGGAGGACGCCGTGTCCTACGTCAGGAAGGT
  GATTCCCAAGGACTACAAAACCATGACTGCGCTGGCCAAGGCCATCTCCAAGAACGTGCTCTTCGCTCAC
  CTGGATGACAACGAGAGGAGTGACATATTCGATGCCATGTTCCCTGTCACTCACATCGCTGGGGAGACTG
  TTATACAGCAAGGGAATGAAGGAGACAACTTCTATGTCGTTGATCAAGGGGAAGTGGATGTGTACGTGAA
  CGGAGAGTGGGTGACCAACATCAGCGAGGGAGGCAGCTTCGGGGAGCTGGCGCTCATCTACGGCACCCCC
  AGGGCTGCGACCGTGAAAGCCAAGACGGACCTCAAGCTCTGGGGGATCGACCGGGACAGCTACCGGCGCA
  TCCTTATGGGCAGCACGCTGAGGAAACGCAAGATGTACGAGGAGTTCCTCAGCAAGGTCTCCATCCTAGA
  GTCCCTGGAGAAGTGGGAGCGTCTGACCGTGGCGGATGCGCTGGAGCCCGTCCAGTTTGAAGATGGAGAG
  AAAATTGTGGTCCAGGGAGAGCCTGGGGACGACTTTTACATCATCACGGAGGGCACCGCGTCCGTGCTGC
  AGCGCCGGTCCCCCAATGAGGAGTACGTGGAGGTGGGGCGCCTGGGACCCTCTGACTACTTCGGGGAGAT
  TGCACTGCTGCTGAACCGGCCCCGGGCGGCCACTGTCGTGGCCCGGGGGCCCCTCAAGTGTGTGAAGCTG
  GACCGGCCCCGCTTCGAGCGTGTGCTGGGGCCCTGCTCTGAGATCCTCAAGAGGAACATTCAGCGTTACA
  ACAGCTTCATCTCCCTCACCGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207809 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MASPPACPSEEDESLKGCELYVQLHGIQQVLKDCIVHLCISKPERPMKFLREHFEKLEKEENRQILARQK
  SNSQSDSHDEEVSPTPPNPVVKARRRRGGVSAEVYTEEDAVSYVRKVIPKDYKTMTALAKAISKNVLFAH
  LDDNERSDIFDAMFPVTHIAGETVIQQGNEGDNFYVVDQGEVDVYVNGEWVTNISEGGSFGELALIYGTP
  RAATVKAKTDLKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLEKWERLTVADALEPVQFEDGE
  KIVVQGEPGDDFYIITEGTASVLQRRSPNEEYVEVGRLGPSDYFGEIALLLNRPRAATVVARGPLKCVKL
  DRPRFERVLGPCSEILKRNIQRYNSFISLTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002735
• ORF Size: 1143 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002735.2
• RefSeq Size: 2512 bp
• RefSeq ORF: 1146 bp
• Locus ID: 5575
• UniProt ID: P31321
• Cytogenetics: 7p22.3
• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Insulin signaling pathway
• MW: 43.1 kDa
• Gene Summary: The protein encoded by this gene is a regulatory subunit of cyclic AMP-dependent protein kinase A (PKA), which is involved in the signaling pathway of the second messenger cAMP. Two regulatory and two catalytic subunits form the PKA holoenzyme, disbands after cAMP binding. The holoenzyme is involved in many cellular events, including ion transport, metabolism, and transcription. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2015]