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TTL (NM_153712) Human Tagged ORF Clone

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SKU
RC207805
TTL (Myc-DDK-tagged)-Human tubulin tyrosine ligase (TTL)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $686.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TTL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207805 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACACCTTCGTGGTACGCGATGAGAACAGCAGCGTCTACGCCGAGGTCTCCCGGCTGCTCCTCGCCA
CCGGCCACTGGAAGAGGCTGCGGCGAGACAACCCCAGATTCAACCTGATGCTGGGAGAGAGGAATCGGCT
GCCCTTCGGGAGACTGGGTCACGAGCCCGGGCTGGTACAGTTGGTGAATTACTACAGGGGTGCTGACAAA
CTGTGTCGCAAAGCTTCTTTAGTGAAGCTAATCAAGACAAGCCCTGAACTGGCTGAGTCCTGCACATGGT
TCCCTGAATCTTATGTGATTTATCCAACCAATCTCAAGACTCCAGTTGCTCCAGCACAGAATGGAATTCA
GCCACCAATCAGTAACTCAAGGACAGATGAAAGAGAATTCTTTCTCGCCTCTTATAACAGAAAGAAAGAG
GATGGAGAGGGCAACGTTTGGATTGCAAAGTCATCAGCCGGTGCCAAAGGTGAAGGCATTCTCATCTCCT
CAGAGGCTTCAGAGCTTCTCGATTTCATAGACAACCAGGGCCAAGTGCACGTGATCCAGAAATATCTTGA
GCACCCTCTGCTGCTTGAGCCAGGTCATCGCAAGTTTGACATTCGAAGCTGGGTCTTGGTGGATCATCAG
TATAATATCTACCTCTATAGAGAGGGTGTGCTTCGGACTGCTTCAGAACCATATCATGTTGATAATTTCC
AAGACAAAACCTGCCATTTGACCAATCACTGCATTCAAAAAGAGTATTCAAAGAACTACGGGAAGTATGA
AGAAGGAAATGAAATGTTCTTCAAGGAGTTCAATCAGTACCTAACAAGTGCTTTGAACATTACCCTAGAA
AGTAGTATCTTACTACAAATCAAACATATAATAAGGAACTGCCTCCTGAGCGTGGAGCCTGCCATTAGCA
CCAAGCACCTCCCTTACCAGAGCTTCCAGCTCTTCGGCTTTGACTTCATGGTCGATGAGGAGCTGAAGGT
GTGGCTCATTGAGGTCAACGGTGCCCCTGCATGTGCTCAGAAGCTCTATGCAGAACTGTGCCAAGGCATC
GTGGACATAGCCATTTCCAGTGTCTTCCCACCCCCAGATGTGGAGCAACCTCAGACCCAGCCAGCTGCCT
TCATCAAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207805 protein sequence
Red=Cloning site Green=Tags(s)

MYTFVVRDENSSVYAEVSRLLLATGHWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLVQLVNYYRGADK
LCRKASLVKLIKTSPELAESCTWFPESYVIYPTNLKTPVAPAQNGIQPPISNSRTDEREFFLASYNRKKE
DGEGNVWIAKSSAGAKGEGILISSEASELLDFIDNQGQVHVIQKYLEHPLLLEPGHRKFDIRSWVLVDHQ
YNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFKEFNQYLTSALNITLE
SSILLQIKHIIRNCLLSVEPAISTKHLPYQSFQLFGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGI
VDIAISSVFPPPDVEQPQTQPAAFIKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153712
ORF Size 1131 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153712.2
RefSeq Size 5181 bp
RefSeq ORF 1134 bp
Locus ID 150465
UniProt ID Q8NG68
Cytogenetics 2q14.1
Domains TTL
MW 43.2 kDa
Summary TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 PubMed 14571137).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_153712" in other vectors (6)
SKU Description Size Price
RC207805L1 Lenti ORF clone of Human tubulin tyrosine ligase (TTL), Myc-DDK-tagged 10 ug
$986.00
RC207805L2 Lenti ORF clone of Human tubulin tyrosine ligase (TTL), mGFP tagged 10 ug
$986.00
RC207805L3 Lenti ORF clone of Human tubulin tyrosine ligase (TTL), Myc-DDK-tagged 10 ug
$986.00
RC207805L4 Lenti ORF clone of Human tubulin tyrosine ligase (TTL), mGFP tagged 10 ug
$986.00
RG207805 TTL (tGFP-tagged) - Human tubulin tyrosine ligase (TTL) 10 ug
$886.00
SC100834 TTL (untagged)-Human tubulin tyrosine ligase (TTL) 10 ug
$2,177.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.