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CILP (NM_003613) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC207801L3
Lenti ORF clone of Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,846.00
3 Weeks*
Size
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CILP
Synonyms CILP-1; HsT18872
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207801).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003613
ORF Size 3552 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003613.2
RefSeq Size 4484 bp
RefSeq ORF 3555 bp
Locus ID 8483
UniProt ID O75339
Cytogenetics 15q22.31
Domains ig, IG, IGc2, tsp_1
Protein Families Druggable Genome, Phosphatase, Secreted Protein
MW 132.6 kDa
Summary Major alterations in the composition of the cartilage extracellular matrix occur in joint disease, such as osteoarthrosis. This gene encodes the cartilage intermediate layer protein (CILP), which increases in early osteoarthrosis cartilage. The encoded protein was thought to encode a protein precursor for two different proteins; an N-terminal CILP and a C-terminal homolog of NTPPHase, however, later studies identified no nucleotide pyrophosphatase phosphodiesterase (NPP) activity. The full-length and the N-terminal domain of this protein was shown to function as an IGF-1 antagonist. An allelic variant of this gene has been associated with lumbar disc disease. provided by RefSeq, Sep 2010
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Documents
Resources
"NM_003613" in other vectors (6)
SKU Description Size Price
RC207801 CILP (Myc-DDK-tagged)-Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP) 10 ug
$1,546.00
RC207801L1 Lenti ORF clone of Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), Myc-DDK-tagged 10 ug
$1,846.00
RC207801L2 Lenti ORF clone of Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), mGFP tagged 10 ug
$1,846.00
RC207801L4 Lenti ORF clone of Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP), mGFP tagged 10 ug
$1,846.00
RG207801 CILP (tGFP-tagged) - Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP) 10 ug
$1,746.00
SC117885 CILP (untagged)-Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (CILP) 10 ug
$1,031.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.