NDST2 (NM_003635) Human Tagged ORF Clone

CAT#: RC207792

NDST2 (Myc-DDK-tagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2)

Product Images

Circular map for RC207792

• 

  Western blot validation of overexpression lysate (Cat# LY418535) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207792 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NDST2
• Synonyms: HSST2; N-HSST 2; NST2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207792 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCAGTTGTGGAAGGTGGTACGCCCAGCTCGGCAGCTGGAACTGCACCGCCTCATACTGCTGCTGA
  TCGCTTTCAGCCTGGGCTCCATGGGCTTCCTGGCTTATTATGTGTCCACCAGCCCTAAGGCCAAGGAACC
  CTTGCCCCTGCCCTTGGGAGACTGCAGCAGCGGTGGGGCAGCTGGTCCTGGCCCTGCACGGCCTCCAGTT
  CCACCTCGGCCCCCCAGGCCTCCAGAGACAGCTCGAACTGAACCCGTGGTCCTTGTGTTTGTGGAGAGTG
  CATACTCACAGCTGGGGCAGGAAATTGTGGCCATCCTGGAGTCTAGTCGTTTTCGTTATAGCACTGAGTT
  GGCACCTGGCCGAGGGGACATGCCCACATTGACTGATAATACCCATGGCCGCTATGTCTTGGTCATTTAT
  GAGAACCTGCTCAAGTATGTCAACCTGGATGCCTGGAGTCGGGAACTGCTAGACCGGTACTGCGTGGAGT
  ATGGTGTGGGCATCATTGGCTTTTTCCGAGCCCACGAGCACAGCCTACTGAGCGCCCAGCTCAAGGGCTT
  TCCCCTTTTTTTACACTCAAACTTGGGGCTCCGGGACTACCAAGTGAATCCTTCTGCCCCGCTACTGCAT
  CTCACACGCCCCAGCCGCCTAGAACCAGGGCCACTGCCTGGTGATGACTGGACCATCTTCCAATCCAATC
  ATAGTACATATGAACCAGTGCTTCTTGCCAGCCTTCGGCCAGCTGAGCCCGCAGTGCCAGGACCAGTTCT
  TCGTCGGGCCCGGCTTCCCACTGTGGTACAGGACCTGGGGCTTCATGATGGCATCCAGCGGGTGCTCTTT
  GGACATGGCCTTTCCTTCTGGCTCCACAAACTTATCTTCGTTGATGCTGTTGCATACCTCACTGGCAAGC
  GCCTCTGCCTGGACCTTGACCGCTACATCTTGGTAGACATCGATGACATCTTTGTGGGCAAGGAAGGGAC
  CCGCATGAAGGTGGCTGATGTTGAGGCTCTGTTGACCACCCAGAACAAACTCAGGACCTTAGTTCCCAAC
  TTCACCTTCAACTTGGGCTTCTCGGGCAAGTTCTATCATACTGGGACAGAGGAGGAGGATGCAGGGGACG
  ACATGCTGCTGAAGCACCGCAAAGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCACACCT
  GTTCCACAATCGCTCCGTGCTGGCTGACCAGATGAGGCTCAACAAACAGTTTGCTCTGGAGCATGGGATT
  CCCACGGACCTGGGGTATGCTGTGGCCCCCCACCACTCGGGTGTGTACCCCATCCACACGCAGCTCTATG
  AGGCCTGGAAATCCGTGTGGGGCATCCAGGTGACCAGCACTGAGGAGTATCCCCATCTCCGCCCTGCCCG
  CTACCGCCGTGGCTTCATTCACAATGGCATTATGGTGCTGCCCCGGCAGACATGTGGCCTCTTCACTCAC
  ACAATCTTCTATAATGAGTATCCTGGAGGCTCTCGTGAACTAGACCGGAGCATCCGAGGTGGAGAGCTCT
  TTCTGACAGTGCTGCTTAATCCGATCAGCATCTTTATGACCCATCTGTCCAATTATGGAAATGACCGGCT
  GGGCCTATACACCTTTGAGAGCTTGGTGCGCTTCCTCCAGTGTTGGACACGGCTGCGCCTACAGACCCTT
  CCTCCTGTCCCACTTGCACAGAAGTACTTTGAACTTTTCCCTCAGGAGCGAAGCCCCCTTTGGCAGAATC
  CCTGTGATGACAAGAGGCACAAAGATATCTGGTCCAAGGAGAAAACCTGTGATCGTCTCCCGAAGTTCCT
  CATTGTGGGACCCCAGAAAACAGGGACTACAGCTATTCACTTCTTCCTGAGCCTGCACCCAGCTGTAACT
  AGCAGCTTCCCTAGCCCCAGCACATTTGAGGAGATTCAGTTCTTCAACAGCCCTAATTACCACAAGGGTA
  TTGACTGGTACATGGATTTCTTCCCTGTTCCTTCCAATGCCAGCACTGATTTCCTATTTGAAAAAAGTGC
  CACCTACTTTGACTCTGAAGTTGTACCACGGCGGGGGGCTGCCCTCCTGCCACGAGCCAAGATCATCACA
  GTGCTCACCAACCCTGCTGACAGGGCCTACTCCTGGTACCAGCATCAGCGAGCCCATGGAGACCCAGTTG
  CTCTGAACTATACCTTCTATCAGGTGATTTCAGCCTCCTCCCAGACCCCTCTGGCACTACGCTCCCTGCA
  GAACCGCTGTCTTGTCCCTGGCTACTATTCTACCCATCTACAACGCTGGCTGACTTACTACCCCTCTGGA
  CAGTTGCTGATTGTGGATGGGCAAGAGCTGCGTACCAACCCAGCAGCCTCAATGGAGAGCATCCAGAAGT
  TCCTGGGTATCACACCCTTTCTGAACTACACACGGACCCTCAGGTTTGATGATGATAAGGGATTTTGGTG
  CCAGGGACTTGAAGGTGGTAAGACTCGCTGTCTAGGCCGGAGCAAAGGCCGGAGGTATCCAGATATGGAC
  ACTGAGTCCCGTCTTTTCCTTACGGATTTTTTCCGGAACCATAATTTGGAGTTGTCGAAGCTGCTGAGCC
  GGCTTGGACAGCCAGTGCCCTCGTGGCTTCGGGAAGAACTGCAGCATTCCAGTCTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207792 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLQLWKVVRPARQLELHRLILLLIAFSLGSMGFLAYYVSTSPKAKEPLPLPLGDCSSGGAAGPGPARPPV
  PPRPPRPPETARTEPVVLVFVESAYSQLGQEIVAILESSRFRYSTELAPGRGDMPTLTDNTHGRYVLVIY
  ENLLKYVNLDAWSRELLDRYCVEYGVGIIGFFRAHEHSLLSAQLKGFPLFLHSNLGLRDYQVNPSAPLLH
  LTRPSRLEPGPLPGDDWTIFQSNHSTYEPVLLASLRPAEPAVPGPVLRRARLPTVVQDLGLHDGIQRVLF
  GHGLSFWLHKLIFVDAVAYLTGKRLCLDLDRYILVDIDDIFVGKEGTRMKVADVEALLTTQNKLRTLVPN
  FTFNLGFSGKFYHTGTEEEDAGDDMLLKHRKEFWWFPHMWSHMQPHLFHNRSVLADQMRLNKQFALEHGI
  PTDLGYAVAPHHSGVYPIHTQLYEAWKSVWGIQVTSTEEYPHLRPARYRRGFIHNGIMVLPRQTCGLFTH
  TIFYNEYPGGSRELDRSIRGGELFLTVLLNPISIFMTHLSNYGNDRLGLYTFESLVRFLQCWTRLRLQTL
  PPVPLAQKYFELFPQERSPLWQNPCDDKRHKDIWSKEKTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVT
  SSFPSPSTFEEIQFFNSPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIIT
  VLTNPADRAYSWYQHQRAHGDPVALNYTFYQVISASSQTPLALRSLQNRCLVPGYYSTHLQRWLTYYPSG
  QLLIVDGQELRTNPAASMESIQKFLGITPFLNYTRTLRFDDDKGFWCQGLEGGKTRCLGRSKGRRYPDMD
  TESRLFLTDFFRNHNLELSKLLSRLGQPVPSWLREELQHSSLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003635
• ORF Size: 2649 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003635.4
• RefSeq Size: 4006 bp
• RefSeq ORF: 2652 bp
• Locus ID: 8509
• UniProt ID: P52849
• Cytogenetics: 10q22.2
• Domains: Sulfotransfer
• Protein Families: Transmembrane
• Protein Pathways: Heparan sulfate biosynthesis, Metabolic pathways
• MW: 100.9 kDa
• Gene Summary: This gene encodes a member of the N-deacetylase/N-sulfotransferase subfamily of the sulfotransferase 1 proteins. The encoded enzyme has dual functions in processing glucosamine and heparin polymers, including N-deacetylation and N-sulfation. The encoded protein may be localized to the Golgi. [provided by RefSeq, Feb 2009]