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PLA2G5 (NM_000929) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC207789L4
Lenti-ORF clone of PLA2G5 (mGFP-tagged)-Human phospholipase A2, group V (PLA2G5)
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $525.00
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Other products for "PLA2G5 or NM_000929"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLA2G5
Synonyms FRFB; GV-PLA2; hVPLA(2); PLA2-10
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207789).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000929
ORF Size 414 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000929.2
RefSeq Size 1911 bp
RefSeq ORF 417 bp
Locus ID 5322
UniProt ID P39877
Cytogenetics 1p36.13
Domains PA2c
Protein Families Druggable Genome, Secreted Protein
Protein Pathways alpha-Linolenic acid metabolism, Arachidonic acid metabolism, Ether lipid metabolism, Fc epsilon RI signaling pathway, Glycerophospholipid metabolism, GnRH signaling pathway, Linoleic acid metabolism, Long-term depression, MAPK signaling pathway, Metabolic pathways, Vascular smooth muscle contraction, VEGF signaling pathway
MW 15.67 kDa
Summary This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000929" in other vectors (6)
SKU Description Size Price
RC207789 PLA2G5 (Myc-DDK-tagged)-Human phospholipase A2, group V (PLA2G5) 10 ug
$225.00
RC207789L1 Lenti-ORF clone of PLA2G5 (Myc-DDK-tagged)-Human phospholipase A2, group V (PLA2G5) 10 ug
$525.00
RC207789L2 Lenti-ORF clone of PLA2G5 (mGFP-tagged)-Human phospholipase A2, group V (PLA2G5) 10 ug
$525.00
RC207789L3 Lenti-ORF clone of PLA2G5 (Myc-DDK-tagged)-Human phospholipase A2, group V (PLA2G5) 10 ug
$525.00
RG207789 PLA2G5 (tGFP-tagged) - Human phospholipase A2, group V (PLA2G5) 10 ug
$425.00
SC119544 PLA2G5 (untagged)-Human phospholipase A2, group V (PLA2G5) 10 ug
$889.00

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