SCTR (NM_002980) Human Tagged ORF Clone

CAT#: RC207786

SCTR (Myc-DDK-tagged)-Human secretin receptor (SCTR)

Product Images

Circular map for RC207786

• 

  Western blot validation of overexpression lysate (Cat# LY418973) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207786 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCTR
• Synonyms: SR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207786 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGTCCCCACCTGTCGCCGCCGCTGCAGCAGCTACTACTGCCGGTGCTGCTCGCCTGCGCCGCGCACT
  CGACTGGAGCCCTTCCCCGACTATGTGACGTGCTACAAGTGCTGTGGGAAGAGCAAGACCAGTGCCTGCA
  GGAACTCTCCAGAGAGCAGACAGGAGACCTGGGCACGGAGCAGCCAGTGCCAGGTTGTGAGGGGATGTGG
  GACAACATAAGCTGCTGGCCCTCTTCTGTGCCGGGCCGGATGGTGGAGGTGGAATGCCCGAGATTCCTCC
  GGATGCTCACCAGCAGAAATGGTTCCTTGTTCCGAAACTGCACACAGGATGGCTGGTCAGAAACCTTCCC
  CAGGCCTAATCTGGCCTGTGGCGTTAATGTGAACGACTCTTCCAACGAGAAGCGGCACTCCTACCTGCTG
  AAGCTGAAAGTCATGTACACCGTGGGCTACAGCTCCTCCCTGGTCATGCTCCTGGTCGCCCTTGGCATCC
  TCTGTGCTTTCCGGAGGCTCCACTGCACTCGCAACTACATCCACATGCACCTGTTCGTGTCCTTCATCCT
  TCGTGCCCTGTCCAACTTCATCAAGGACGCCGTGCTCTTCTCCTCAGATGATGTCACCTACTGCGATGCC
  CACAGGGCGGGCTGCAAGCTGATCATGGTGCTGTTCCAGTACTGCATCATGGCCAACTACTCCTGGCTGC
  TGGTGGAAGGCCTCTACCTTCACACACTCCTCGCCATCTCCTTCTTCTCTGAAAGAAAGTACCTCCAGGG
  ATTTGTGGCATTCGGATGGGGTTCTCCAGCCATTTTTGTTGCTTTGTGGGCTATTGCCAGACACTTTCTG
  GAAGATGTTGGGTGCTGGGACATCAATGCCAACGCATCCATCTGGTGGATCATTCGTGGTCCTGTGATCC
  TCTCCATCCTGATTAATTTCATCCTTTTCATAAACATTCTAAGAATCCTGATGAGAAAACTTAGAACCCA
  AGAAACAAGAGGAAATGAAGTCAGCCATTATAAGCGCCTGGCCAGGTCCACTCTCCTGCTGATCCCCCTC
  TTTGGCATCCACTACATCGTCTTCGCCTTCTCCCCAGAGGACGCTATGGAGATCCAGCTGTTTTTTGAAC
  TAGCCCTTGGCTCATTCCAGGGACTGGTGGTGGCCGTCCTCTACTGCTTCCTCAACGGGGAGGTGCAGCT
  GGAGGTTCAGAAGAAGTGGCAGCAATGGCACCTCCGTGAGTTCCCACTGCACCCCGTGGCCTCCTTCAGC
  AACAGCACCAAGGCCAGCCACTTGGAGCAGAGCCAGGGCACCTGCAGGACCAGCATCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207786 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRPHLSPPLQQLLLPVLLACAAHSTGALPRLCDVLQVLWEEQDQCLQELSREQTGDLGTEQPVPGCEGMW
  DNISCWPSSVPGRMVEVECPRFLRMLTSRNGSLFRNCTQDGWSETFPRPNLACGVNVNDSSNEKRHSYLL
  KLKVMYTVGYSSSLVMLLVALGILCAFRRLHCTRNYIHMHLFVSFILRALSNFIKDAVLFSSDDVTYCDA
  HRAGCKLIMVLFQYCIMANYSWLLVEGLYLHTLLAISFFSERKYLQGFVAFGWGSPAIFVALWAIARHFL
  EDVGCWDINANASIWWIIRGPVILSILINFILFINILRILMRKLRTQETRGNEVSHYKRLARSTLLLIPL
  FGIHYIVFAFSPEDAMEIQLFFELALGSFQGLVVAVLYCFLNGEVQLEVQKKWQQWHLREFPLHPVASFS
  NSTKASHLEQSQGTCRTSII
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002980
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002980.3
• RefSeq Size: 1833 bp
• RefSeq ORF: 1323 bp
• Locus ID: 6344
• UniProt ID: P47872
• Cytogenetics: 2q14.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 50.2 kDa
• Gene Summary: The protein encoded by this gene is a G protein-coupled receptor and belongs to the glucagon-VIP-secretin receptor family. It binds secretin which is the most potent regulator of pancreatic bicarbonate, electrolyte and volume secretion. Secretin and its receptor are suggested to be involved in pancreatic cancer and autism. [provided by RefSeq, Jul 2008]