TIE1 (NM_005424) Human Tagged ORF Clone

CAT#: RC207771

TIE1 (Myc-DDK-tagged)-Human tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1)

Product Images

Circular map for RC207771

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TIE1 (Cat# RC207771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIE1(Cat# TA590602). Positive lysates LY417318 (100ug) and LC417318 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY417318) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207771 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TIE1 protein (Cat# TP307771). The protein was produced from HEK293T cells transfected with TIE1 cDNA clone (Cat# RC207771) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TIE1
• Synonyms: JTK14; LMPHM11; TIE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207771 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGGCGGGTGCCCCCTTTCTTGCTCCCCATCCTCTTCTTGGCTTCTCATGTGGGCGCGGCGGTGG
  ACCTGACGCTGCTGGCCAACCTGCGGCTCACGGACCCCCAGCGCTTCTTCCTGACTTGCGTGTCTGGGGA
  GGCCGGGGCGGGGAGGGGCTCGGACGCCTGGGGCCCGCCCCTGCTGCTGGAGAAGGACGACCGTATCGTG
  CGCACCCCGCCCGGGCCACCCCTGCGCCTGGCGCGCAACGGTTCGCACCAGGTCACGCTTCGCGGCTTCT
  CCAAGCCCTCGGACCTCGTGGGCGTCTTCTCCTGCGTGGGCGGTGCTGGGGCGCGGCGCACGCGCGTCAT
  CTACGTGCACAACAGCCCTGGAGCCCACCTGCTTCCAGACAAGGTCACACACACTGTGAACAAAGGTGAC
  ACCGCTGTACTTTCTGCACGTGTGCACAAGGAGAAGCAGACAGACGTGATCTGGAAGAGCAACGGATCCT
  ACTTCTACACCCTGGACTGGCATGAAGCCCAGGATGGGCGGTTCCTGCTGCAGCTCCCAAATGTGCAGCC
  ACCATCGAGCGGCATCTACAGTGCCACTTACCTGGAAGCCAGCCCCCTGGGCAGCGCCTTCTTTCGGCTC
  ATCGTGCGGGGTTGTGGGGCTGGGCGCTGGGGGCCAGGCTGTACCAAGGAGTGCCCAGGTTGCCTACATG
  GAGGTGTCTGCCACGACCATGACGGCGAATGTGTATGCCCCCCTGGCTTCACTGGCACCCGCTGTGAACA
  GGCCTGCAGAGAGGGCCGTTTTGGGCAGAGCTGCCAGGAGCAGTGCCCAGGCATATCAGGCTGCCGGGGC
  CTCACCTTCTGCCTCCCAGACCCCTATGGCTGCTCTTGTGGATCTGGCTGGAGAGGAAGCCAGTGCCAAG
  AAGCTTGTGCCCCTGGTCATTTTGGGGCTGATTGCCGACTCCAGTGCCAGTGTCAGAATGGTGGCACTTG
  TGACCGGTTCAGTGGTTGTGTCTGCCCCTCTGGGTGGCATGGAGTGCACTGTGAGAAGTCAGACCGGATC
  CCCCAGATCCTCAACATGGCCTCAGAACTGGAGTTCAACTTAGAGACGATGCCCCGGATCAACTGTGCAG
  CTGCAGGGAACCCCTTCCCCGTGCGGGGCAGCATAGAGCTACGCAAGCCAGACGGCACTGTGCTCCTGTC
  CACCAAGGCCATTGTGGAGCCAGAGAAGACCACAGCTGAGTTCGAGGTGCCCCGCTTGGTTCTTGCGGAC
  AGTGGGTTCTGGGAGTGCCGTGTGTCCACATCTGGCGGCCAAGACAGCCGGCGCTTCAAGGTCAATGTGA
  AAGTGCCCCCCGTGCCCCTGGCTGCACCTCGGCTCCTGACCAAGCAGAGCCGCCAGCTTGTGGTCTCCCC
  GCTGGTCTCGTTCTCTGGGGATGGACCCATCTCCACTGTCCGCCTGCACTACCGGCCCCAGGACAGTACC
  ATGGACTGGTCGACCATTGTGGTGGACCCCAGTGAGAACGTGACGTTAATGAACCTGAGGCCAAAGACAG
  GATACAGTGTTCGTGTGCAGCTGAGCCGGCCAGGGGAAGGAGGAGAGGGGGCCTGGGGGCCTCCCACCCT
  CATGACCACAGACTGTCCTGAGCCTTTGTTGCAGCCGTGGTTGGAGGGCTGGCATGTGGAAGGCACTGAC
  CGGCTGCGAGTGAGCTGGTCCTTGCCCTTGGTGCCCGGGCCACTGGTGGGCGACGGTTTCCTGCTGCGCC
  TGTGGGACGGGACACGGGGGCAGGAGCGGCGGGAGAACGTCTCATCCCCCCAGGCCCGCACTGCCCTCCT
  GACGGGACTCACGCCTGGCACCCACTACCAGCTGGATGTGCAGCTCTACCACTGCACCCTCCTGGGCCCG
  GCCTCGCCCCCTGCACACGTGCTTCTGCCCCCCAGTGGGCCTCCAGCCCCCCGACACCTCCACGCCCAGG
  CCCTCTCAGACTCCGAGATCCAGCTGACATGGAAGCACCCGGAGGCTCTGCCTGGGCCAATATCCAAGTA
  CGTTGTGGAGGTGCAGGTGGCTGGGGGTGCAGGAGACCCACTGTGGATAGACGTGGACAGGCCTGAGGAG
  ACAAGCACCATCATCCGTGGCCTCAACGCCAGCACGCGCTACCTCTTCCGCATGCGGGCCAGCATTCAGG
  GGCTCGGGGACTGGAGCAACACAGTAGAAGAGTCCACCCTGGGCAACGGGCTGCAGGCTGAGGGCCCAGT
  CCAAGAGAGCCGGGCAGCTGAAGAGGGCCTGGATCAGCAGCTGATCCTGGCGGTGGTGGGCTCCGTGTCT
  GCCACCTGCCTCACCATCCTGGCCGCCCTTTTAACCCTGGTGTGCATCCGCAGAAGCTGCCTGCATCGGA
  GACGCACCTTCACCTACCAGTCAGGCTCGGGCGAGGAGACCATCCTGCAGTTCAGCTCAGGGACCTTGAC
  ACTTACCCGGCGGCCAAAACTGCAGCCCGAGCCCCTGAGCTACCCAGTGCTAGAGTGGGAGGACATCACC
  TTTGAGGACCTCATCGGGGAGGGGAACTTCGGCCAGGTCATCCGGGCCATGATCAAGAAGGACGGGCTGA
  AGATGAACGCAGCCATCAAAATGCTGAAAGAGTATGCCTCTGAAAATGACCATCGTGACTTTGCGGGAGA
  ACTGGAAGTTCTGTGCAAATTGGGGCATCACCCCAACATCATCAACCTCCTGGGGGCCTGTAAGAACCGA
  GGTTACTTGTATATCGCTATTGAATATGCCCCCTACGGGAACCTGCTAGATTTTCTGCGGAAAAGCCGGG
  TCCTAGAGACTGACCCAGCTTTTGCTCGAGAGCATGGGACAGCCTCTACCCTTAGCTCCCGGCAGCTGCT
  GCGTTTCGCCAGTGATGCGGCCAATGGCATGCAGTACCTGAGTGAGAAGCAGTTCATCCACAGGGACCTG
  GCTGCCCGGAATGTGCTGGTCGGAGAGAACCTAGCCTCCAAGATTGCAGACTTCGGCCTTTCTCGGGGAG
  AGGAGGTTTATGTGAAGAAGACGATGGGGCGTCTCCCTGTGCGCTGGATGGCCATTGAGTCCCTGAACTA
  CAGTGTCTATACCACCAAGAGTGATGTCTGGTCCTTTGGAGTCCTTCTTTGGGAGATAGTGAGCCTTGGA
  GGTACACCCTACTGTGGCATGACCTGTGCCGAGCTCTATGAAAAGCTGCCCCAGGGCTACCGCATGGAGC
  AGCCTCGAAACTGTGACGATGAAGTGTACGAGCTGATGCGTCAGTGCTGGCGGGACCGTCCCTATGAGCG
  ACCCCCCTTTGCCCAGATTGCGCTACAGCTAGGCCGCATGCTGGAAGCCAGGAAGGCCTATGTGAACATG
  TCGCTGTTTGAGAACTTCACTTACGCGGGCATTGATGCCACAGCTGAGGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207771 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVWRVPPFLLPILFLASHVGAAVDLTLLANLRLTDPQRFFLTCVSGEAGAGRGSDAWGPPLLLEKDDRIV
  RTPPGPPLRLARNGSHQVTLRGFSKPSDLVGVFSCVGGAGARRTRVIYVHNSPGAHLLPDKVTHTVNKGD
  TAVLSARVHKEKQTDVIWKSNGSYFYTLDWHEAQDGRFLLQLPNVQPPSSGIYSATYLEASPLGSAFFRL
  IVRGCGAGRWGPGCTKECPGCLHGGVCHDHDGECVCPPGFTGTRCEQACREGRFGQSCQEQCPGISGCRG
  LTFCLPDPYGCSCGSGWRGSQCQEACAPGHFGADCRLQCQCQNGGTCDRFSGCVCPSGWHGVHCEKSDRI
  PQILNMASELEFNLETMPRINCAAAGNPFPVRGSIELRKPDGTVLLSTKAIVEPEKTTAEFEVPRLVLAD
  SGFWECRVSTSGGQDSRRFKVNVKVPPVPLAAPRLLTKQSRQLVVSPLVSFSGDGPISTVRLHYRPQDST
  MDWSTIVVDPSENVTLMNLRPKTGYSVRVQLSRPGEGGEGAWGPPTLMTTDCPEPLLQPWLEGWHVEGTD
  RLRVSWSLPLVPGPLVGDGFLLRLWDGTRGQERRENVSSPQARTALLTGLTPGTHYQLDVQLYHCTLLGP
  ASPPAHVLLPPSGPPAPRHLHAQALSDSEIQLTWKHPEALPGPISKYVVEVQVAGGAGDPLWIDVDRPEE
  TSTIIRGLNASTRYLFRMRASIQGLGDWSNTVEESTLGNGLQAEGPVQESRAAEEGLDQQLILAVVGSVS
  ATCLTILAALLTLVCIRRSCLHRRRTFTYQSGSGEETILQFSSGTLTLTRRPKLQPEPLSYPVLEWEDIT
  FEDLIGEGNFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNR
  GYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDL
  AARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLG
  GTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNM
  SLFENFTYAGIDATAEEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005424
• ORF Size: 3414 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005424.2, NP_005415.1
• RefSeq Size: 4000 bp
• RefSeq ORF: 3417 bp
• Locus ID: 7075
• UniProt ID: P35590
• Cytogenetics: 1p34.2
• Domains: pkinase, TyrKc, S_TKc, ig, IG, FN3, EGF, EGF
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• MW: 125.1 kDa
• Gene Summary: This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]