ST3GAL2 (NM_006927) Human Tagged ORF Clone

CAT#: RC207761

ST3GAL2 (Myc-DDK-tagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2)

Product Images

Circular map for RC207761

• 

  Western blot validation of overexpression lysate (Cat# LY416319) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207761 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ST3GAL2
• Synonyms: Gal-NAc6S; SIAT4B; ST3GalA.2; ST3GALII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207761 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGTGCTCCCTGCGGGTGTGGTTCCTCTCCGTGGCCTTCCTGCTGGTGTTCATCATGTCCCTGCTCT
  TCACCTACTCGCACCACAGCATGGCCACGCTCCCCTACCTGGACTCAGGGGCCCTGGATGGGACGCACCG
  GGTGAAGCTGGTGCCCGGCTATGCCGGCCTGCAGCGCCTCAGCAAGGAGAGGCTCTCGGGCAAGAGCTGT
  GCCTGTCGCCGCTGCATGGGCGATGCCGGTGCCTCCGACTGGTTTGACAGCCACTTTGACGGTAACATTT
  CCCCCGTCTGGACCCGAGAGAACATGGATCTTCCACCGGACGTCCAGAGGTGGTGGATGATGCTGCAGCC
  CCAGTTCAAGTCACACAACACCAATGAGGTGCTGGAGAAGCTGTTCCAGATAGTGCCTGGCGAGAACCCC
  TACCGCTTCCGGGACCCCCACCAGTGCCGGCGCTGTGCCGTGGTGGGGAACTCGGGCAACCTGCGGGGCT
  CTGGCTATGGGCAGGACGTGGACGGGCACAACTTCATCATGAGGATGAATCAGGCGCCAACCGTGGGCTT
  TGAGCAGGATGTTGGCAGCCGAACCACCCACCATTTCATGTACCCTGAGAGTGCCAAGAACCTGCCCGCC
  AACGTCAGCTTCGTGCTGGTGCCCTTCAAGGTCCTGGACCTTCTGTGGATCGCCAGCGCCTTGTCCACGG
  GGCAGATCCGATTCACCTACGCCCCAGTGAAGTCCTTCCTTCGAGTGGATAAAGAAAAGGTCCAGATCTA
  CAACCCAGCCTTCTTCAAGTATATCCACGACAGGTGGACAGAGCATCACGGGCGGTACCCTTCCACGGGG
  ATGCTGGTGCTTTTCTTTGCCCTGCATGTGTGTGATGAGGTGAACGTGTACGGGTTCGGGGCCGACAGCC
  GGGGCAACTGGCACCACTACTGGGAGAACAACCGGTACGCGGGCGAGTTCCGGAAGACTGGCGTGCACGA
  CGCGGACTTCGAGGCCCACATCATCGACATGCTGGCCAAGGCCAGCAAGATCGAAGTCTACCGGGGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207761 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKCSLRVWFLSVAFLLVFIMSLLFTYSHHSMATLPYLDSGALDGTHRVKLVPGYAGLQRLSKERLSGKSC
  ACRRCMGDAGASDWFDSHFDGNISPVWTRENMDLPPDVQRWWMMLQPQFKSHNTNEVLEKLFQIVPGENP
  YRFRDPHQCRRCAVVGNSGNLRGSGYGQDVDGHNFIMRMNQAPTVGFEQDVGSRTTHHFMYPESAKNLPA
  NVSFVLVPFKVLDLLWIASALSTGQIRFTYAPVKSFLRVDKEKVQIYNPAFFKYIHDRWTEHHGRYPSTG
  MLVLFFALHVCDEVNVYGFGADSRGNWHHYWENNRYAGEFRKTGVHDADFEAHIIDMLAKASKIEVYRGN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006927
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006927.4
• RefSeq Size: 4450 bp
• RefSeq ORF: 1053 bp
• Locus ID: 6483
• UniProt ID: Q16842
• Cytogenetics: 16q22.1
• Domains: Glyco_transf_29
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Keratan sulfate biosynthesis, Metabolic pathways, O-Glycan biosynthesis
• MW: 40.2 kDa
• Gene Summary: The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4A. [provided by RefSeq, Jul 2008]