Encephalopsin (OPN3) (NM_014322) Human Tagged ORF Clone

CAT#: RC207753

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OPN3 (Myc-DDK-tagged)-Human opsin 3 (OPN3)



  "NM_014322" in other vectors (5)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol OPN3
Synonyms ECPN; PPP1R116
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207753 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACTCGGGGAACCGCAGCGGCGGCCACGGCTACTGGGACGGCGGCGGGGCCGCGGGCGCTGAGGGGC
CGGCGCCGGCGGGGACACTGAGCCCCGCGCCCCTCTTCAGCCCCGGCACCTACGAGCGCCTGGCGCTGCT
GCTGGGCTCCATTGGGCTGCTGGGCGTCGGCAACAACCTGCTGGTGCTCGTCCTCTACTACAAGTTCCAG
CGGCTCCGCACTCCCACTCACCTCCTCCTGGTCAACATCAGCCTCAGCGACCTGCTGGTGTCCCTCTTCG
GGGTCACCTTTACCTTCGTGTCCTGCCTGAGGAACGGCTGGGTGTGGGACACCGTGGGCTGCGTGTGGGA
CGGGTTTAGCGGCAGCCTCTTCGGGATTGTTTCCATTGCCACCCTAACCGTGCTGGCCTATGAACGTTAC
ATTCGCGTGGTCCATGCCAGAGTGATCAATTTTTCCTGGGCCTGGAGGGCCATTACCTACATCTGGCTCT
ACTCACTGGCGTGGGCAGGAGCACCTCTCCTGGGATGGAACAGGTACATCCTGGACGTACACGGACTAGG
CTGCACTGTGGACTGGAAATCCAAGGATGCCAACGATTCCTCCTTTGTGCTTTTCTTATTTCTTGGCTGC
CTGGTGGTGCCCCTGGGTGTCATAGCCCATTGCTATGGCCATATTCTATATTCCATTCGAATGCTTCGTT
GTGTGGAAGATCTTCAGACAATTCAAGTGATCAAGATTTTAAAATATGAAAAGAAACTGGCCAAAATGTG
CTTTTTAATGATATTCACCTTCCTGGTCTGTTGGATGCCTTATATCGTGATCTGCTTCTTGGTGGTTAAT
GGTCATGGTCACCTGGTCACTCCAACAATATCTATTGTTTCGTACCTCTTTGCTAAATCGAACACTGTAT
ACAATCCAGTGATTTATGTCTTCATGATCAGAAAGTTTCGAAGATCCCTTTTGCAGCTTCTGTGCCTCCG
ACTGCTGAGGTGCCAGAGGCCTGCTAAAGACCTACCAGCAGCTGGAAGTGAAATGCAGATCAGACCCATT
GTGATGTCACAGAAAGATGGGGACAGGCCAAAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTA
TCATCACCAGTGATGAATCACTGTCAGTTGACGACAGCGACAAAACCAATGGGTCCAAAGTTGATGTAAT
CCAAGTTCGTCCTTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207753 protein sequence
Red=Cloning site Green=Tags(s)

MYSGNRSGGHGYWDGGGAAGAEGPAPAGTLSPAPLFSPGTYERLALLLGSIGLLGVGNNLLVLVLYYKFQ
RLRTPTHLLLVNISLSDLLVSLFGVTFTFVSCLRNGWVWDTVGCVWDGFSGSLFGIVSIATLTVLAYERY
IRVVHARVINFSWAWRAITYIWLYSLAWAGAPLLGWNRYILDVHGLGCTVDWKSKDANDSSFVLFLFLGC
LVVPLGVIAHCYGHILYSIRMLRCVEDLQTIQVIKILKYEKKLAKMCFLMIFTFLVCWMPYIVICFLVVN
GHGHLVTPTISIVSYLFAKSNTVYNPVIYVFMIRKFRRSLLQLLCLRLLRCQRPAKDLPAAGSEMQIRPI
VMSQKDGDRPKKKVTFNSSSIIFIITSDESLSVDDSDKTNGSKVDVIQVRPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014322
ORF Size 1206 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014322.3
RefSeq Size 2632 bp
RefSeq ORF 1209 bp
Locus ID 23596
UniProt ID Q9H1Y3
Cytogenetics 1q43
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
MW 44.9 kDa
Gene Summary Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. In addition to the visual opsins, mammals possess several photoreceptive non-visual opsins that are expressed in extraocular tissues. This gene, opsin 3, is strongly expressed in brain and testis and weakly expressed in liver, placenta, heart, lung, skeletal muscle, kidney, and pancreas. The gene may also be expressed in the retina. The protein has the canonical features of a photoreceptive opsin protein. [provided by RefSeq, Jul 2008]

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