Talin 1 (TLN1) (NM_006289) Human Tagged Lenti ORF Clone
$2,397.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Talin 1 |
| Synonyms | ILWEQ; talin-1; TLN |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_006289 |
| ORF Size | 7623 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006289.2 |
| RefSeq Size | 8187 bp |
| RefSeq ORF | 7626 bp |
| Locus ID | 7094 |
| UniProt ID | Q9Y490 |
| Cytogenetics | 9p13.3 |
| Domains | B41, I_LWEQ |
| Protein Families | Druggable Genome |
| Protein Pathways | Focal adhesion |
| MW | 269.6 kDa |
| Summary | This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. provided by RefSeq, Feb 2009 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Plasmid Map for RC207713L1
Download sequence for
Cloning scheme for RC207713L1
Circular map for RC207713L1
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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