POLE4 (NM_019896) Human Tagged ORF Clone
CAT#: RC207681
POLE4 (Myc-DDK-tagged)-Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_019896" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | POLE4 |
Synonyms | p12; YHHQ1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207681 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGCGGCAGGAAGCGGGACGCCCCGAGAGGAGGAGGTACCTGCTGGGGAGGCAGCGG CCTCGCAGCCCCAGGCCCCAACGAGTGTGCCTGGGGCTCGTCTCTCGAGGTTGCCTCTGGCGCGAGTGAA GGCCTTGGTGAAGGCAGATCCCGACGTGACGCTAGCGGGACAGGAAGCCATCTTCATTCTGGCACGAGCC GCGGAACTGTTTGTGGAGACCATTGCAAAAGATGCCTACTGTTGCGCTCAGCAGGGAAAAAGGAAAACCC TTCAGAGGAGAGACTTGGATAATGCAATAGAAGCTGTGGATGAATTTGCTTTTCTGGAAGGTACTTTAGA T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207681 protein sequence
Red=Cloning site Green=Tags(s) MAAAAAAGSGTPREEEVPAGEAAASQPQAPTSVPGARLSRLPLARVKALVKADPDVTLAGQEAIFILARA AELFVETIAKDAYCCAQQGKRKTLQRRDLDNAIEAVDEFAFLEGTLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_019896 |
ORF Size | 351 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019896.1 |
RefSeq Size | 710 bp |
RefSeq ORF | 354 bp |
Locus ID | 56655 |
UniProt ID | Q9NR33 |
Cytogenetics | 2p12 |
Domains | CBFD_NFYB_HMF |
Protein Pathways | Base excision repair, DNA replication, Metabolic pathways, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism |
MW | 12.3 kDa |
Gene Summary | POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.[supplied by OMIM, Apr 2004] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207681L3 | Lenti ORF clone of Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4), Myc-DDK-tagged |
USD 450.00 |
|
RC207681L4 | Lenti ORF clone of Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4), mGFP tagged |
USD 450.00 |
|
RG207681 | POLE4 (tGFP-tagged) - Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) |
USD 350.00 |
|
SC108865 | POLE4 (untagged)-Human polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) |
USD 150.00 |
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