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SEPTIN12 (NM_144605) Human Tagged ORF Clone

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SKU
RC207655
SEPT1 (Myc-DDK-tagged)-Human septin 12 (SEPT12), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Size
Bulk Requests & Clone Modifications

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Other products for "SEPTIN12 or NM_144605"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SEPTIN12
Synonyms SEPT12; SPGF10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207655 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACCCCCTGAGGCGCTCCCCCTCTCCCTGCCTGTCCTCGCAGCCCTCCAGCCCCAGCACCCCACCCT
GCGAGATGCTTGGTCCTGTGGGCATTGAGGCTGTGCTGGACCAGCTGAAGATCAAGGCTATGAAGATGGG
GTTTGAGTTCAACATCATGGTGGTGGGGCAAAGCGGGCTGGGCAAGTCCACGATGGTGAACACGCTGTTC
AAGTCCAAAGTGTGGAAGTCAAACCCACCGGGCTTGGGGGTGCCCACACCCCAGACGCTGCAGCTGCATT
CACTGACCCATGTCATAGAGGAGAAGGGTGTGAAGCTGAAGCTGACGGTGACGGACACGCCCGGCTTCGG
GGACCAGATCAACAATGACAACTGCTGGGACCCCATCCTGGGCTACATCAACGAGCAATACGAGCAGTAC
CTGCAGGAGGAGATCCTCATCACCCGCCAGCGCCACATCCCAGACACCCGGGTGCACTGCTGCGTGTACT
TTGTACCACCCACTGGGCACTGCCTGCGGCCCCTGGACATTGAGTTCCTGCAGCGGCTGTGCCGGACTGT
GAATGTGGTGCCCGTGATTGCCAGGGCCGACAGCCTGACCATGGAGGAGCGAGAGGCCTTCAGGCGCAGG
ATCCAGCAGAACCTGAGGACCCACTGCATCGACGTCTACCCCCAGATGTGCTTTGACGAGGACATCAATG
ACAAAATCCTCAACAGCAAGTTACGGGACCGAATCCCTTTTGCCGTGGTAGGGGCTGACCAAGAGCACCT
GGTGAACGGGAGGTGTGTCCTGGGCCGGAAGACCAAGTGGGGCATCATTGAAGTGGAGAACATGGCGCAC
TGTGAATTTCCTCTCCTGAGAGACCTGCTTATCCGCTCCCACCTCCAAGACCTGAAGGACATAACCCACA
ACATCCACTATGAGAACTACCGCGTCATCAGACTCAATGAAAGCCACCTGCTGCCCCGCGGGCCCGGCTG
GGTGAACCTGGCCCCGGCCTCCCCAGGACAGCTGACCACCCCCCGGACCTTCAAGGTCTGCAGGGGGGCC
CATGACGATTCTGATGATGAGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207655 protein sequence
Red=Cloning site Green=Tags(s)

MDPLRRSPSPCLSSQPSSPSTPPCEMLGPVGIEAVLDQLKIKAMKMGFEFNIMVVGQSGLGKSTMVNTLF
KSKVWKSNPPGLGVPTPQTLQLHSLTHVIEEKGVKLKLTVTDTPGFGDQINNDNCWDPILGYINEQYEQY
LQEEILITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLQRLCRTVNVVPVIARADSLTMEEREAFRRR
IQQNLRTHCIDVYPQMCFDEDINDKILNSKLRDRIPFAVVGADQEHLVNGRCVLGRKTKWGIIEVENMAH
CEFPLLRDLLIRSHLQDLKDITHNIHYENYRVIRLNESHLLPRGPGWVNLAPASPGQLTTPRTFKVCRGA
HDDSDDEF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144605
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144605.5
RefSeq Size 1524 bp
RefSeq ORF 1077 bp
Locus ID 124404
UniProt ID Q8IYM1
Cytogenetics 16p13.3
MW 40.7 kDa
Summary This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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Resources
"NM_144605" in other vectors (4)
SKU Description Size Price
RC207655L3 Lenti ORF clone of Human septin 12 (SEPT12), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC207655L4 Lenti ORF clone of Human septin 12 (SEPT12), transcript variant 2, mGFP tagged 10 ug
$757.00
RG207655 41529 (tGFP-tagged) - Human septin 12 (SEPT12), transcript variant 2 10 ug
$657.00
SC123172 41529 (untagged)-Human septin 12 (SEPT12), transcript variant 2 10 ug
$457.00

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