TLK1 (NM_012290) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207653L2
Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, mGFP tagged
  $1,001.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TLK1
Synonyms PKU-beta
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207653).
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_012290
ORF Size 2298 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012290.3, NP_036422.3
RefSeq Size 5751 bp
RefSeq ORF 2301 bp
Locus ID 9874
UniProt ID Q9UKI8
Cytogenetics 2q31.1
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
MW 86.7 kDa
Summary The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
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Documents
Resources
"NM_012290" in other vectors (6)
SKU Description Size Price
RC207653 TLK1 (Myc-DDK-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 1 10 ug
$701.00
RC207653L1 Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,001.00
RC207653L3 Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,001.00
RC207653L4 Lenti ORF clone of Human tousled-like kinase 1 (TLK1), transcript variant 1, mGFP tagged 10 ug
$1,001.00
RG207653 TLK1 (tGFP-tagged) - Human tousled-like kinase 1 (TLK1), transcript variant 1 10 ug
$789.00 $901.00
SC108453 TLK1 (untagged)-Human tousled-like kinase 1 (TLK1), transcript variant 1 10 ug
$702.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.