Zinc Alpha 2 Glycoprotein (AZGP1) (NM_001185) Human Tagged ORF Clone

SKU
RC207625
AZGP1 (Myc-DDK-tagged)-Human alpha-2-glycoprotein 1, zinc-binding (AZGP1)
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$300.00
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Specifications
Product Data
Target Symbol Zinc Alpha 2 Glycoprotein
Synonyms ZA2G; ZAG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207625 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAAGAATGGTGCCTGTCCTGCTGTCTCTGCTGCTGCTTCTGGGTCCTGCTGTCCCCCAGGAGAACC
AAGATGGTCGTTACTCTCTGACCTATATCTACACTGGGCTGTCCAAGCATGTTGAAGACGTCCCCGCGTT
TCAGGCCCTTGGCTCACTCAATGACCTCCAGTTCTTTAGATACAACAGTAAAGACAGGAAGTCTCAGCCC
ATGGGACTCTGGAGACAGGTGGAAGGAATGGAGGATTGGAAGCAGGACAGCCAACTTCAGAAGGCCAGGG
AGGACATCTTTATGGAGACCCTGAAAGACATTGTGGAGTATTACAACGACAGTAACGGGTCTCACGTATT
GCAGGGAAGGTTTGGTTGTGAGATCGAGAATAACAGAAGCAGCGGAGCATTCTGGAAATATTACTATGAT
GGAAAGGACTACATTGAATTCAACAAAGAAATCCCAGCCTGGGTCCCCTTCGACCCAGCAGCCCAGATAA
CCAAGCAGAAGTGGGAGGCAGAACCAGTCTACGTGCAGCGGGCCAAGGCTTACCTGGAGGAGGAGTGCCC
TGCGACTCTGCGGAAATACCTGAAATACAGCAAAAATATCCTGGACCGGCAAGATCCTCCCTCTGTGGTG
GTCACCAGCCACCAGGCCCCAGGAGAAAAGAAGAAACTGAAGTGCCTGGCCTACGACTTCTACCCAGGGA
AAATTGATGTGCACTGGACTCGGGCCGGCGAGGTGCAGGAGCCTGAGTTACGGGGAGATGTTCTTCACAA
TGGAAATGGCACTTACCAGTCCTGGGTGGTGGTGGCAGTGCCCCCGCAGGACACAGCCCCCTACTCCTGC
CACGTGCAGCACAGCAGCCTGGCCCAGCCCCTCGTGGTGCCCTGGGAGGCCAGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207625 protein sequence
Red=Cloning site Green=Tags(s)

MVRMVPVLLSLLLLLGPAVPQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQP
MGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYD
GKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVV
VTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSC
HVQHSSLAQPLVVPWEAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001185
ORF Size 894 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001185.1
RefSeq Size 1278 bp
RefSeq ORF 897 bp
Locus ID 563
UniProt ID P25311
Cytogenetics 7q22.1
Domains ig, IGc1, MHC_I
Protein Families Secreted Protein
MW 34.3 kDa
Summary Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
RC207625L1 Lenti ORF clone of Human alpha-2-glycoprotein 1, zinc-binding (AZGP1), Myc-DDK-tagged 10 ug
$600.00
RC207625L2 Lenti ORF clone of Human alpha-2-glycoprotein 1, zinc-binding (AZGP1), mGFP tagged 10 ug
$600.00
RC207625L3 Lenti ORF clone of Human alpha-2-glycoprotein 1, zinc-binding (AZGP1), Myc-DDK-tagged 10 ug
$600.00
RC207625L4 Lenti ORF clone of Human alpha-2-glycoprotein 1, zinc-binding (AZGP1), mGFP tagged 10 ug
$600.00
RG207625 AZGP1 (tGFP-tagged) - Human alpha-2-glycoprotein 1, zinc-binding (AZGP1) 10 ug
$489.00 MSRP $500.00 MSRP $500.00
SC321752 AZGP1 (untagged)-Human alpha-2-glycoprotein 1, zinc-binding (AZGP1) 10 ug
$300.00

Citations

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