GABA A Receptor delta (GABRD) (NM_000815) Human Tagged Lenti ORF Clone
- LentiORF®
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | GABA A Receptor delta |
| Synonyms | EIG10; EJM7; GEFSP5 |
| Vector | pLenti-C-Myc-DDK |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_000815 |
| ORF Size | 1356 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000815.2 |
| RefSeq Size | 1942 bp |
| RefSeq ORF | 1359 bp |
| Locus ID | 2563 |
| UniProt ID | O14764 |
| Cytogenetics | 1p36.33 |
| Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 50.7 kDa |
| Summary | Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. The GABA-A receptor is generally pentameric and there are five types of subunits: alpha, beta, gamma, delta, and rho. This gene encodes the delta subunit. Mutations in this gene have been associated with susceptibility to generalized epilepsy with febrile seizures, type 5. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000815" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207591 | GABRD (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD) | 10 ug |
$289.00
$457.00
|
|
| RC207591L2 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD), mGFP tagged | 10 ug |
$757.00
|
|
| RC207591L3 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC207591L4 | Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD), mGFP tagged | 10 ug |
$757.00
|
|
| RG207591 | GABRD (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD) | 10 ug |
$489.00
$657.00
|
|
| SC122576 | GABRD (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD) | 10 ug |
$457.00
|
|
Plasmid Map for RC207591L1
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Cloning scheme for RC207591L1
Circular map for RC207591L1
Citations
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