GABA A Receptor delta (GABRD) (NM_000815) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207591L1
Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD), Myc-DDK-tagged
  $757.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GABA A Receptor delta
Synonyms EIG10; EJM7; GEFSP5
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207591).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000815
ORF Size 1356 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000815.2
RefSeq Size 1942 bp
RefSeq ORF 1359 bp
Locus ID 2563
UniProt ID O14764
Cytogenetics 1p36.33
Protein Families Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 50.7 kDa
Summary Gamma-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. The GABA-A receptor is generally pentameric and there are five types of subunits: alpha, beta, gamma, delta, and rho. This gene encodes the delta subunit. Mutations in this gene have been associated with susceptibility to generalized epilepsy with febrile seizures, type 5. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_000815" in other vectors (6)
SKU Description Size Price
RC207591 GABRD (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD) 10 ug
$289.00 $457.00
RC207591L2 Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD), mGFP tagged 10 ug
$757.00
RC207591L3 Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD), Myc-DDK-tagged 10 ug
$757.00
RC207591L4 Lenti ORF clone of Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD), mGFP tagged 10 ug
$757.00
RG207591 GABRD (tGFP-tagged) - Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD) 10 ug
$489.00 $657.00
SC122576 GABRD (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, delta (GABRD) 10 ug
$457.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.