BOB1 (POU2AF1) (NM_006235) Human Tagged ORF Clone

CAT#: RC207562

POU2AF1 (Myc-DDK-tagged)-Human POU class 2 associating factor 1 (POU2AF1)

Product Images

Circular map for RC207562

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY POU2AF1 (Cat# RC207562, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1 (Cat# TA807930)(1:2000). Positive lysates LY416779 (100ug) and LC416779 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416779) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207562 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BOB1
• Synonyms: BOB1; OBF-1; OBF1; OCAB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207562 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTGGCAAAAACCCACAGCTCCGGAGCAAGCCCCAGCCCCGGCCCGGCCATACCAGGGCGTCCGTG
  TGAAGGAGCCAGTGAAGGAACTGCTGAGGAGGAAGCGAGGCCACGCCAGCAGTGGGGCAGCACCTGCACC
  TACGGCGGTGGTGCTGCCCCATCAGCCCCTGGCGACCTACACCACAGTGGGTCCTTCCTGCCTGGACATG
  GAAGGTTCTGTGTCTGCAGTGACAGAGGAGGCTGCCCTGTGTGCCGGCTGGCTCTCCCAGCCCACCCCGG
  CCACCCTGCAGCCCCTGGCCCCATGGACACCTTACACCGAGTATGTGCCCCATGAAGCTGTCAGCTGCCC
  CTACTCAGCTGACATGTATGTGCAGCCCGTGTGCCCCAGCTACACGGTGGTGGGGCCCTCCTCAGTGTTG
  ACCTATGCCTCTCCGCCACTCATCACCAATGTCACGACAAGAAGCTCCGCCACGCCCGCAGTGGGGCCCC
  CGCTGGAGGGCCCAGAGCACCAGGCACCCCTCACCTATTTCCCGTGGCCTCAGCCCCTTTCCACACTACC
  CACCTCCACCCTGCAGTACCAGCCTCCGGCCCCAGCCCTACCTGGGCCCCAGTTTGTCCAGCTCCCCATC
  TCTATCCCAGAGCCAGTCCTTCAGGACATGGAAGACCCCAGAAGAGCCGCCAGCTCGTTGACCATCGACA
  AGCTGCTTTTGGAGGAAGAGGATAGCGACGCCTATGCGCTTAACCACACTCTCTCTGTGGAAGGCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207562 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLWQKPTAPEQAPAPARPYQGVRVKEPVKELLRRKRGHASSGAAPAPTAVVLPHQPLATYTTVGPSCLDM
  EGSVSAVTEEAALCAGWLSQPTPATLQPLAPWTPYTEYVPHEAVSCPYSADMYVQPVCPSYTVVGPSSVL
  TYASPPLITNVTTRSSATPAVGPPLEGPEHQAPLTYFPWPQPLSTLPTSTLQYQPPAPALPGPQFVQLPI
  SIPEPVLQDMEDPRRAASSLTIDKLLLEEEDSDAYALNHTLSVEGF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006235
• ORF Size: 768 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006235.3
• RefSeq Size: 3032 bp
• RefSeq ORF: 771 bp
• Locus ID: 5450
• UniProt ID: Q16633
• Cytogenetics: 11q23.1
• MW: 27.4 kDa
• Gene Summary: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.[UniProtKB/Swiss-Prot Function]