Peripherin (PRPH) (NM_006262) Human Tagged ORF Clone
CAT#: RC207561
PRPH (Myc-DDK-tagged)-Human peripherin (PRPH)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
AAV Particle: DDK
"NM_006262" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Peripherin |
Synonyms | NEF4; PRPH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207561 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCACCACCCGTCGGGCCTCCGGGCCGGCTTCAGCTCCACCTCATACCGCCGTACCTTCGGTCCAC CGCCCTCACTATCCCCCGGGGCCTTCTCCTACTCGTCCAGCTCCCGCTTCTCCAGCAGCCGCCTGCTGGG CTCCGCGTCCCCGAGCTCCTCGGTGCGCCTGGGCAGCTTCCGTAGCCCCCGAGCGGGAGCGGGCGCCCTC CTGCGCCTGCCCTCGGAGCGCCTCGACTTCTCCATGGCCGAGGCCCTCAACCAGGAGTTCCTGGCCACGC GCAGCAACGAGAAGCAGGAGCTGCAGGAGCTCAACGACCGCTTCGCCAACTTCATCGAGAAGGTACGCTT TCTGGAGCAGCAGAACGCGGCCCTGCGCGGGGAGCTGAGCCAAGCCCGGGGCCAGGAGCCGGCGCGCGCC GACCAGCTGTGCCAGCAGGAGCTGCGCGAGCTGCGGCGAGAGCTGGAGCTGTTGGGCCGCGAGCGTGACC GGGTGCAGGTGGAGCGCGACGGGCTGGCGGAGGACCTGGCGGCGCTCAAGCAGAGGTTGGAGGAGGAGAC GCGCAAGCGGGAGGACGCGGAGCACAACCTCGTGCTCTTCCGCAAGGACGTGGACGATGCCACTCTGTCC CGCCTGGAACTAGAGCGCAAGATTGAGTCTCTGATGGATGAGATTGAGTTCCTCAAGAAGCTGCACGAGG AGGAGCTGCGAGACCTGCAGGTGAGTGTGGAGAGCCAGCAGGTGCAGCAGGTGGAGGTGGAAGCCACGGT GAAGCCCGAGCTGACGGCAGCGCTGAGGGACATCCGCGCGCAGTACGAGAGCATCGCCGCGAAGAACCTG CAGGAGGCGGAGGAGTGGTACAAGTCCAAGTACGCGGACCTGTCCGACGCTGCCAACCGGAACCACGAGG CCCTGCGCCAGGCCAAGCAGGAGATGAACGAGTCCCGACGCCAGATCCAGAGTCTAACGTGCGAGGTGGA CGGGCTGCGCGGCACGAACGAGGCGCTGCTCAGGCAGTTGAGAGAGCTGGAGGAGCAGTTCGCCCTGGAG GCGGGGGGCTACCAGGCGGGCGCTGCGCGGCTCGAGGAGGAGCTGCGACAGCTAAAAGAGGAGATGGCGC GGCACCTGAGGGAGTACCAGGAGCTCCTCAACGTCAAGATGGCCCTGGACATCGAGATCGCCACCTACCG CAAGCTGCTGGAGGGCGAGGAGAGCCGGATCTCCGTGCCCGTCCATTCTTTTGCCTCCTTAAATATAAAG ACGACTGTGCCTGAGGTGGAGCCTCCCCAGGACAGCCACAGCCGGAAGACGGTTCTGATCAAGACCATTG AGACCCGGAATGGGGAGGTGGTGACAGAGTCCCAGAAGGAGCAGCGCAGTGAGCTGGACAAGTCTTCTGC CCACAGTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207561 protein sequence
Red=Cloning site Green=Tags(s) MSHHPSGLRAGFSSTSYRRTFGPPPSLSPGAFSYSSSSRFSSSRLLGSASPSSSVRLGSFRSPRAGAGAL LRLPSERLDFSMAEALNQEFLATRSNEKQELQELNDRFANFIEKVRFLEQQNAALRGELSQARGQEPARA DQLCQQELRELRRELELLGRERDRVQVERDGLAEDLAALKQRLEEETRKREDAEHNLVLFRKDVDDATLS RLELERKIESLMDEIEFLKKLHEEELRDLQVSVESQQVQQVEVEATVKPELTAALRDIRAQYESIAAKNL QEAEEWYKSKYADLSDAANRNHEALRQAKQEMNESRRQIQSLTCEVDGLRGTNEALLRQLRELEEQFALE AGGYQAGAARLEEELRQLKEEMARHLREYQELLNVKMALDIEIATYRKLLEGEESRISVPVHSFASLNIK TTVPEVEPPQDSHSRKTVLIKTIETRNGEVVTESQKEQRSELDKSSAHSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_006262 |
ORF Size | 1410 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006262.4 |
RefSeq Size | 1833 bp |
RefSeq ORF | 1413 bp |
Locus ID | 5630 |
UniProt ID | P41219 |
Cytogenetics | 12q13.12 |
Protein Pathways | Amyotrophic lateral sclerosis (ALS) |
MW | 53.7 kDa |
Gene Summary | This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207561L1 | Lenti ORF clone of Human peripherin (PRPH), Myc-DDK-tagged |
USD 757.00 |
|
RC207561L2 | Lenti ORF clone of Human peripherin (PRPH), mGFP tagged |
USD 757.00 |
|
RC207561L3 | Lenti ORF clone of Human peripherin (PRPH), Myc-DDK-tagged |
USD 757.00 |
|
RC207561L4 | Lenti ORF clone of Human peripherin (PRPH), mGFP tagged |
USD 757.00 |
|
RG207561 | PRPH (tGFP-tagged) - Human peripherin (PRPH) |
USD 657.00 |
|
SC123831 | PRPH (untagged)-Human peripherin (PRPH) |
USD 457.00 |
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