Bim (BCL2L11) (NM_138621) Human Tagged ORF Clone
BCL2L11 (Myc-DDK-tagged)-Human BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 1
|Product Name||Bim (BCL2L11) (NM_138621) Human Tagged ORF Clone|
|Type||Human Tagged ORF Clone|
|Synonyms||BAM; BIM; BOD|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC207559 representing NM_138621
Red=Cloning site Blue=ORF Green=Tags(s)
>RC207559 representing NM_138621
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||AscI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||594 bp|
|OTI Disclaimer||The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info|
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq||NM_138621.1, NM_138621.2, NM_138621.3, NM_138621.4, NP_619527.1|
|RefSeq Size||3422 bp|
|RefSeq ORF||597 bp|
|Protein Families||Druggable Genome|
|Gene Summary||The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013]|
|The use of this cDNA Clones has been cited in the following citations:|
Antitumor activity of S116836, a novel tyrosine kinase inhibitor, against imatinib-resistant FIP1L1-PDGFRa-expressing cells
,Shen, Y;Ren, X;Ding, K;Zhang, Z;Wang, D;Pan, J;,
Oncotarget Jun 2014
Role for the Proapoptotic Factor BIM in Mediating Imatinib-induced Apoptosis in a c-KIT-dependent Gastrointestinal Stromal Tumor Cell Line
,Peter M. Gordon and David E. Fisher,
J. Biol. Chem., May 2010; 285: 14109 - 14114
|cDNA Clone Resources|
|SC306048||BCL2L11 (untagged)-Human BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 1||
|RG207559||BCL2L11 (GFP-tagged) - Human BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 1||
|RC207559L1||Lenti ORF clone of Human BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 1, Myc-DDK-tagged||
|RC207559L2||Lenti ORF clone of Human BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 1, mGFP tagged||
|RC207559L3||Lenti ORF clone of Human BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 1, Myc-DDK-tagged||
|RC207559L4||Lenti ORF clone of Human BCL2-like 11 (apoptosis facilitator) (BCL2L11), transcript variant 1, mGFP tagged||