TGDS (NM_014305) Human Tagged ORF Clone

CAT#: RC207534

TGDS (Myc-DDK-tagged)-Human TDP-glucose 4,6-dehydratase (TGDS)

Product Images

Circular map for RC207534

• 

  Western blot validation of overexpression lysate (Cat# LY415374) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207534 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TGDS
• Synonyms: CATMANS; SDR2E1; TDPGD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207534 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCGGCGTGTTGGGAGGAACCGTGGGGTCTTCCCGGCGGCTTTGCGAAGCGGGTCCTGGTGACCG
  GCGGTGCTGGTTTCATTGCATCACATATGATTGTCTCTTTAGTGGAAGATTATCCAAACTATATGATCAT
  AAATCTAGACAAGCTGGATTACTGTGCAAGCTTGAAGAATCTTGAAACCATTTCTAACAAACAGAACTAC
  AAATTTATACAGGGTGACATATGTGATTCTCACTTTGTGAAACTGCTTTTTGAAACAGAGAAAATAGATA
  TAGTACTACATTTTGCCGCACAAACACATGTAGATCTTTCATTCGTACGTGCCTTTGAGTTTACCTATGT
  TAATGTTTATGGCACTCACGTTTTGGTAAGTGCTGCTCATGAAGCCAGAGTGGAGAAGTTTATTTATGTC
  AGCACAGATGAAGTATATGGTGGCAGTCTTGATAAGGAATTTGATGAATCTTCACCCAAACAACCTACAA
  ATCCTTATGCATCATCTAAAGCAGCTGCTGAATGTTTTGTACAGTCTTACTGGGAACAATATAAGTTTCC
  AGTTGTCATCACAAGAAGCAGTAATGTTTATGGACCACATCAATATCCAGAAAAGGTTATTCCAAAATTT
  ATATCTTTGCTACAGCACAACAGGAAATGTTGCATTCATGGGTCAGGGCTTCAAACAAGAAACTTCCTTT
  ATGCTACTGATGTTGTAGAAGCATTTCTCACTGTCCTCAAAAAAGGGAAACCAGGTGAAATTTATAACAT
  CGGAACCAATTTTGAAATGTCAGTTGTCCAGCTTGCCAAAGAACTAATACAACTGATCAAAGAGACCAAT
  TCAGAGTCTGAAATGGAAAATTGGGTTGATTATGTTAATGATAGACCCACCAATGACATGAGATACCCAA
  TGAAGTCAGAAAAAATACATGGCTTAGGATGGAGACCTAAAGTGCCTTGGAAAGAAGGAATAAAGAAAAC
  AATTGAATGGTACAGAGAGAATTTTCACAACTGGAAGAATGTGGAAAAGGCATTAGAACCCTTTCCGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207534 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAACWEEPWGLPGGFAKRVLVTGGAGFIASHMIVSLVEDYPNYMIINLDKLDYCASLKNLETISNKQNY
  KFIQGDICDSHFVKLLFETEKIDIVLHFAAQTHVDLSFVRAFEFTYVNVYGTHVLVSAAHEARVEKFIYV
  STDEVYGGSLDKEFDESSPKQPTNPYASSKAAAECFVQSYWEQYKFPVVITRSSNVYGPHQYPEKVIPKF
  ISLLQHNRKCCIHGSGLQTRNFLYATDVVEAFLTVLKKGKPGEIYNIGTNFEMSVVQLAKELIQLIKETN
  SESEMENWVDYVNDRPTNDMRYPMKSEKIHGLGWRPKVPWKEGIKKTIEWYRENFHNWKNVEKALEPFPV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014305
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014305.4
• RefSeq Size: 1952 bp
• RefSeq ORF: 1053 bp
• Locus ID: 23483
• UniProt ID: O95455
• Cytogenetics: 13q32.1
• Domains: Epimerase
• MW: 40.2 kDa
• Gene Summary: The protein encoded by this gene is a member of the short-chain dehydrogenases/reductases (SDR) superfamily, and is thought to contain a nicotinamide adenine dinucleotide (NAD) binding domain. This large SDR family of enzymes is involved in the metabolism of a variety of compounds, including prostaglandins, retinoids, lipids, steroid hormones, and xenobiotics. Mutations in this gene have been associated with Catel-Manzke syndrome, which is characterized by Pierre Robin sequence, and radial deviation of the index finger due to the presence of an accessory bone between the index finger and its proximal phalanx. Pierre Robin sequence is defined by an undersized jaw, backwards displacement of the tongue base that causes an obstruction of the airways, and can also be associated with a cleft palate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]