BET1L (NM_016526) Human Tagged ORF Clone

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SKU
RC207516
BET1L (Myc-DDK-tagged)-Human blocked early in transport 1 homolog (S. cerevisiae)-like (BET1L), transcript variant 2
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BET1L
Synonyms BET1L1; GOLIM3; GS15; HSPC197
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207516 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACTGGGCTCGGGCTCAGAGCCCGGGCGCTGTGGAAGAGATTCTAGACCGGGAGAACAAGCGAA
TGGCTGACAGCCTGGCCTCCAAAGTCACCAGGCTCAAATCGCTCGCCCTGGACATCGATAGGGATGCAGA
GGATCAGAACCGGTACCTGGATGGCATGGTAAGGGCCCACGGTGTGCGTGTATCAGTGCCCTGCCCTAGC
ACTACCTGCTGCAGAGCCTGCAGTGTCTCCTTCAGCACTGGTGCGGGTGGGTGGTCAAATCACCACTTCT
GTGTGATCTTGCTGGGATTCCTCCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207516 protein sequence
Red=Cloning site Green=Tags(s)

MADWARAQSPGAVEEILDRENKRMADSLASKVTRLKSLALDIDRDAEDQNRYLDGMVRAHGVRVSVPCPS
TTCCRACSVSFSTGAGGWSNHHFCVILLGFLP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016526
ORF Size 306 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016526.4
RefSeq Size 2971 bp
RefSeq ORF 309 bp
Locus ID 51272
Cytogenetics 11p15.5
Protein Families Transmembrane
Protein Pathways SNARE interactions in vesicular transport
MW 11.1 kDa
Summary Vesicle SNARE required for targeting and fusion of retrograde transport vesicles with the Golgi complex. Required for the integrity of the Golgi complex (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016526" in other vectors (6)
SKU Description Size Price
RC207516L1 Lenti ORF clone of Human blocked early in transport 1 homolog (S. cerevisiae)-like (BET1L), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC207516L2 Lenti ORF clone of Human blocked early in transport 1 homolog (S. cerevisiae)-like (BET1L), transcript variant 2, mGFP tagged 10 ug
$450.00
RC207516L3 Lenti ORF clone of Human blocked early in transport 1 homolog (S. cerevisiae)-like (BET1L), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC207516L4 Lenti ORF clone of Human blocked early in transport 1 homolog (S. cerevisiae)-like (BET1L), transcript variant 2, mGFP tagged 10 ug
$450.00
RG207516 BET1L (tGFP-tagged) - Human blocked early in transport 1 homolog (S. cerevisiae)-like (BET1L), transcript variant 2 10 ug
$489.00
SC114193 BET1L (untagged)-Human blocked early in transport 1 homolog (S. cerevisiae)-like (BET1L), transcript variant 2 10 ug
$150.00

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