TLR9 (NM_017442) Human Tagged Lenti ORF Clone
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | TLR9 |
| Synonyms | CD289 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_017442 |
| ORF Size | 3096 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_017442.2 |
| RefSeq Size | 3868 bp |
| RefSeq ORF | 3099 bp |
| Locus ID | 54106 |
| UniProt ID | Q9NR96 |
| Cytogenetics | 3p21.2 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Toll-like receptor signaling pathway |
| MW | 115.86 kDa |
| Summary | The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. provided by RefSeq, Aug 2017 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_017442" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207510 | TLR9 (Myc-DDK-tagged)-Human toll-like receptor 9 (TLR9) | 10 ug |
$589.00
$898.00
|
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| RC207510L1 | Lenti ORF clone of Human toll-like receptor 9 (TLR9), Myc-DDK-tagged | 10 ug |
$1,198.00
|
|
| RC207510L2 | Lenti ORF clone of Human toll-like receptor 9 (TLR9), mGFP tagged | 10 ug |
$1,198.00
|
|
| RC207510L3 | Lenti ORF clone of Human toll-like receptor 9 (TLR9), Myc-DDK-tagged | 10 ug |
$1,198.00
|
|
| RG207510 | TLR9 (tGFP-tagged) - Human toll-like receptor 9 (TLR9) | 10 ug |
$789.00
$1,098.00
|
|
| SC122858 | TLR9 (untagged)-Human toll-like receptor 9 (TLR9) | 10 ug |
$899.00
|
|
Plasmid Map for RC207510L4
Download sequence for
Cloning scheme for RC207510L4
Circular map for RC207510L4
Citations
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