DIP13B (APPL2) (NM_018171) Human Tagged ORF Clone

CAT#: RC207506

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APPL2 (Myc-DDK-tagged)-Human adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 2 (APPL2)



  "NM_018171" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 544.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol APPL2
Synonyms DIP13B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207506 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCGCCGTGGACAAGCTCCTGCTAGAGGAGGCGTTGCAGGACAGCCCCCAGACTCGCTCTTTACTGA
GCGTGTTTGAAGAAGATGCTGGCACCCTCACAGACTATACCAACCAGCTGCTCCAGGCAATGCAGCGCGT
CTATGGAGCCCAGAATGAGATGTGCCTGGCCACACAACAGCTTTCTAAGCAACTGCTGGCATATGAAAAA
CAGAACTTTGCTCTTGGCAAAGGTGATGAAGAAGTAATTTCAACACTCCACTATTTTTCCAAAGTGGTGG
ATGAGCTTAATCTTCTCCATACAGAGCTGGCTAAACAGTTGGCAGACACAATGGTTCTACCTATCATACA
ATTCCGAGAAAAGGATCTCACAGAAGTAAGCACTTTAAAGGATCTATTTGGACTCGCTAGCAATGAGCAT
GACCTCTCAATGGCAAAATACAGCAGGCTGCCTAAGAAAAAGGAGAATGAGAAGGTGAAGACCGAAGTCG
GAAAAGAGGTGGCCGCGGCCCGGCGGAAGCAGCATCTCTCCTCCCTTCAGTACTACTGTGCCCTCAACGC
GCTGCAGTACAGAAAGCAAATGGCCATGATGGAGCCCATGATAGGCTTTGCCCATGGACAGATTAACTTT
TTTAAGAAGGGAGCAGAGATGTTTTCCAAACGTATGGACAGCTTTTTATCCTCCGTTGCAGACATGGTTC
AAAGCATTCAGGTAGAACTGGAAGCCGAGGCGGAAAAGATGCGGGTGTCCCAGCAAGAATTACTTTCTGT
TGATGAATCTGTTTACACTCCAGACTCTGATGTGGCCGCACCACAGATCAACAGGAACCTCATCCAGAAG
GCTGGTTACCTTAATCTTAGAAACAAAACAGGGCTGGTCACCACCACCTGGGAGAGGCTTTATTTCTTCA
CCCAAGGCGGGAATCTCATGTGTCAGCCCAGGGGAGCCGTGGCTGGAGGTTTGATCCAGGACCTGGACAA
CTGCTCAGTGATGGCCGTGGATTGCGAAGACCGGCGCTACTGCTTCCAGATCACCACGCCCAATGGAAAA
TCGGGAATAATCCTCCAGGCTGAGAGCAGAAAGGAAAATGAAGAGTGGATATGTGCAATAAACAACATCT
CCAGACAGATCTACCTGACCGACAACCCTGAGGCAGTCGCGATCAAGTTGAATCAGACCGCTCTGCAAGC
AGTGACTCCCATTACAAGTTTTGGAAAAAAACAAGAAAGCTCATGCCCCAGCCAGAACCTGAAAAATTCA
GAGATGGAAAATGAAAATGACAAGATTGTTCCCAAAGTAACAGCCAGTCTACCTGAAGCAGAGGAGCTGA
TCGCGCCTGGAACGCCGATTCAATTCGATATTGTGCTTCCTGCTACAGAATTCCTTGATCAGAACAGAGG
GAGCAGGCGTACCAACCCTTTTGGTGAAACTGAGGATGAATCATTTCCAGAAGCAGAAGATTCTCTTTTG
CAGCAGATGTTTATAGTTCGGTTTTTGGGATCAATGGCAGTTAAAACAGACAGCACTACTGAAGTGATTT
ATGAAGCGATGAGACAAGTATTGGCTGCTCGGGCTATTCATAACATCTTCCGCATGACAGAATCCCATCT
GATGGTCACCAGTCAATCTTTGAGGTTGATAGATCCACAGACTCAAGTATCAAGGGCCAATTTTGAACTT
ACCAGTGTCACACAATTTGCTGCTCATCAAGAAAACAAGAGACTGGTTGGTTTTGTCATCCGTGTTCCTG
AATCCACTGGAGAAGAATCTCTGAGTACATACATTTTTGAAAGCAACTCAGAAGGCGAAAAGATATGTTA
TGCTATTAATTTGGGAAAAGAAATTATTGAGGTTCAGAAGGATCCAGAAGCACTGGCTCAATTAATGCTG
TCCATACCACTAACCAATGATGGAAAATATGTACTGTTAAACGATCAACCAGATGACGATGATGGAAATC
CAAATGAACATAGAGGCGCAGAATCCGAAGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207506 protein sequence
Red=Cloning site Green=Tags(s)

MPAVDKLLLEEALQDSPQTRSLLSVFEEDAGTLTDYTNQLLQAMQRVYGAQNEMCLATQQLSKQLLAYEK
QNFALGKGDEEVISTLHYFSKVVDELNLLHTELAKQLADTMVLPIIQFREKDLTEVSTLKDLFGLASNEH
DLSMAKYSRLPKKKENEKVKTEVGKEVAAARRKQHLSSLQYYCALNALQYRKQMAMMEPMIGFAHGQINF
FKKGAEMFSKRMDSFLSSVADMVQSIQVELEAEAEKMRVSQQELLSVDESVYTPDSDVAAPQINRNLIQK
AGYLNLRNKTGLVTTTWERLYFFTQGGNLMCQPRGAVAGGLIQDLDNCSVMAVDCEDRRYCFQITTPNGK
SGIILQAESRKENEEWICAINNISRQIYLTDNPEAVAIKLNQTALQAVTPITSFGKKQESSCPSQNLKNS
EMENENDKIVPKVTASLPEAEELIAPGTPIQFDIVLPATEFLDQNRGSRRTNPFGETEDESFPEAEDSLL
QQMFIVRFLGSMAVKTDSTTEVIYEAMRQVLAARAIHNIFRMTESHLMVTSQSLRLIDPQTQVSRANFEL
TSVTQFAAHQENKRLVGFVIRVPESTGEESLSTYIFESNSEGEKICYAINLGKEIIEVQKDPEALAQLML
SIPLTNDGKYVLLNDQPDDDDGNPNEHRGAESEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_018171
ORF Size 1992 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_018171.5
RefSeq Size 3289 bp
RefSeq ORF 1995 bp
Locus ID 55198
UniProt ID Q8NEU8
Cytogenetics 12q23.3
Domains PH, PID
MW 74.5 kDa
Gene Summary The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome. [provided by RefSeq, Oct 2011]

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