CYP20A1 (NM_177538) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207488L3
Lenti ORF clone of Human cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1), Myc-DDK-tagged
  $757.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CYP20A1
Synonyms CYP-M
Vector pLenti-C-Myc-DDK-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207488).
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_177538
ORF Size 1386 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177538.1
RefSeq Size 10943 bp
RefSeq ORF 1389 bp
Locus ID 57404
UniProt ID Q6UW02
Cytogenetics 2q33.2
Protein Families Druggable Genome, P450, Transmembrane
MW 52.5 kDa
Summary This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein lacks one amino acid of the conserved heme binding site. It also lacks the conserved I-helix motif AGX(D,E)T, suggesting that its substrate may carry its own oxygen. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_177538" in other vectors (6)
SKU Description Size Price
RC207488 CYP20A1 (Myc-DDK-tagged)-Human cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1) 10 ug
$457.00
RC207488L1 Lenti ORF clone of Human cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1), Myc-DDK-tagged 10 ug
$757.00
RC207488L2 Lenti ORF clone of Human cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1), mGFP tagged 10 ug
$757.00
RC207488L4 Lenti ORF clone of Human cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1), mGFP tagged 10 ug
$757.00
RG207488 CYP20A1 (tGFP-tagged) - Human cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1) 10 ug
$489.00 $657.00
SC321733 CYP20A1 (untagged)-Human cytochrome P450, family 20, subfamily A, polypeptide 1 (CYP20A1) 10 ug
$457.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.