Neuroglobin (NGB) (NM_021257) Human Tagged ORF Clone

CAT#: RC207482

NGB (Myc-DDK-tagged)-Human neuroglobin (NGB)

Product Images

Western blot validation of overexpression lysate (Cat# LY402862) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207482 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NGB protein (Cat# TP307482). The protein was produced from HEK293T cells transfected with NGB cDNA clone (Cat# RC207482) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NGB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207482 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGCCCGGAGCCCGAGCTGATCCGGCAGAGCTGGCGGGCAGTGAGCCGCAGCCCGCTGGAGCACG
  GCACCGTCCTGTTTGCCAGGCTGTTTGCCCTGGAGCCTGACCTGCTGCCCCTCTTCCAGTACAACTGCCG
  CCAGTTCTCCAGCCCAGAGGACTGTCTCTCCTCGCCTGAGTTCCTGGACCACATCAGGAAGGTGATGCTC
  GTGATTGATGCTGCAGTGACCAATGTGGAAGACCTGTCCTCACTGGAGGAGTACCTTGCCAGCCTGGGCA
  GGAAGCACCGGGCAGTGGGTGTGAAGCTCAGCTCCTTCTCGACAGTGGGTGAGTCTCTGCTCTACATGCT
  GGAGAAGTGTCTGGGCCCTGCCTTCACACCAGCCACACGGGCTGCCTGGAGCCAACTCTACGGGGCCGTA
  GTGCAGGCCATGAGTCGAGGCTGGGATGGCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207482 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERPEPELIRQSWRAVSRSPLEHGTVLFARLFALEPDLLPLFQYNCRQFSSPEDCLSSPEFLDHIRKVML
  VIDAAVTNVEDLSSLEEYLASLGRKHRAVGVKLSSFSTVGESLLYMLEKCLGPAFTPATRAAWSQLYGAV
  VQAMSRGWDGE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021257
• ORF Size: 453 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021257.3, NP_067080.1
• RefSeq Size: 1885 bp
• RefSeq ORF: 456 bp
• Locus ID: 58157
• UniProt ID: Q9NPG2
• Cytogenetics: 14q24.3
• MW: 16.9 kDa
• Gene Summary: This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008]