CTRP4 (C1QTNF4) (NM_031909) Human Tagged ORF Clone

CAT#: RC207458

C1QTNF4 (Myc-DDK-tagged)-Human C1q and tumor necrosis factor related protein 4 (C1QTNF4)

Product Images

Circular map for RC207458

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  Western blot validation of overexpression lysate (Cat# LY410444) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207458 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified C1QTNF4 protein (Cat# TP307458). The protein was produced from HEK293T cells transfected with C1QTNF4 cDNA clone (Cat# RC207458) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTRP4
• Synonyms: CTRP4; ZACRP4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207458 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCGCTTCTGCTGGGCCTGCTGGGCCCAGCGGCCTGCTGGGCCCTGGGCCCGACCCCCGGCCCGG
  GATCCTCTGAGCTGCGCTCGGCCTTCTCGGCGGCACGCACCACCCCCCTGGAGGGCACGTCGGAGATGGC
  GGTGACCTTCGACAAGGTGTACGTGAACATCGGGGGCGACTTCGATGTGGCCACCGGCCAGTTTCGCTGC
  CGCGTGCCCGGCGCCTACTTCTTCTCCTTCACGGCTGGCAAGGCCCCGCACAAGAGCCTGTCGGTGATGC
  TGGTGCGAAACCGCGACGAGGTGCAGGCGCTGGCCTTCGACGAGCAGCGGCGGCCAGGCGCGCGGCGCGC
  AGCCAGCCAGAGCGCCATGCTGCAGCTCGACTACGGCGACACAGTGTGGCTGCGGCTGCATGGCGCCCCG
  CAGTACGCGCTAGGCGCGCCCGGCGCCACCTTCAGCGGCTACCTAGTCTACGCCGACGCCGACGCTGACG
  CGCCTGCGCGCGGGCCGCCCGCGCCCCCCGAGCCGCGCTCGGCCTTCTCGGCGGCGCGCACGCGCAGCTT
  GGTGGGCTCGGACGCTGGCCCCGGGCCGCGGCACCAACCACTCGCCTTCGACACCGAGTTCGTCAACATT
  GGCGGCGACTTCGACGCGGCGGCCGGCGTGTTCCGCTGCCGTCTGCCCGGCGCCTACTTCTTCTCCTTCA
  CGCTGGGCAAGCTGCCGCGTAAGACGCTGTCGGTTAAGCTGATGAAGAACCGCGACGAGGTGCAGGCCAT
  GATTTACGACGACGGCGCGTCGCGGCGCCGCGAGATGCAGAGCCAGAGCGTGATGCTGGCCCTGCGGCGC
  GGCGACGCCGTCTGGCTGCTCAGCCACGACCACGACGGCTACGGCGCCTACAGCAACCACGGCAAGTACA
  TCACCTTCTCCGGCTTCCTGGTGTACCCCGACCTCGCCCCCGCCGCCCCGCCGGGCCTCGGGGCCTCGGA
  GCTACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207458 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLPLLLGLLGPAACWALGPTPGPGSSELRSAFSAARTTPLEGTSEMAVTFDKVYVNIGGDFDVATGQFRC
  RVPGAYFFSFTAGKAPHKSLSVMLVRNRDEVQALAFDEQRRPGARRAASQSAMLQLDYGDTVWLRLHGAP
  QYALGAPGATFSGYLVYADADADAPARGPPAPPEPRSAFSAARTRSLVGSDAGPGPRHQPLAFDTEFVNI
  GGDFDAAAGVFRCRLPGAYFFSFTLGKLPRKTLSVKLMKNRDEVQAMIYDDGASRRREMQSQSVMLALRR
  GDAVWLLSHDHDGYGAYSNHGKYITFSGFLVYPDLAPAAPPGLGASELL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031909
• ORF Size: 987 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031909.3
• RefSeq Size: 1436 bp
• RefSeq ORF: 990 bp
• Locus ID: 114900
• UniProt ID: Q9BXJ3
• Cytogenetics: 11p11.2
• Domains: C1Q
• Protein Families: Secreted Protein
• MW: 35.3 kDa
• Gene Summary: May be involved in the regulation of the inflammatory network. Its role as pro- or anti-inflammatory seems to be context dependent (PubMed:21658842, PubMed:27086950). Seems to have some role in regulating food intake and energy balance when administered in the brain. This effect is sustained over a two-day period, and it is accompanied by decreased expression of orexigenic neuropeptides in the hypothalamus 3 h post-injection (By similarity).[UniProtKB/Swiss-Prot Function]