CBR4 (NM_032783) Human Tagged Lenti ORF Clone
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | CBR4 |
| Synonyms | SDR45C1 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_032783 |
| ORF Size | 711 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_032783.3 |
| RefSeq Size | 3523 bp |
| RefSeq ORF | 714 bp |
| Locus ID | 84869 |
| UniProt ID | Q8N4T8 |
| Cytogenetics | 4q32.3 |
| Domains | adh_short |
| Protein Families | Druggable Genome |
| MW | 25.3 kDa |
| Summary | The heterotetramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis (PubMed:19571038, PubMed:25203508). Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD (PubMed:25203508). The homotetramer has NADPH-dependent quinone reductase activity (PubMed:19000905). Both homotetramer and the heterotetramer have broad substrate specificity and can reduce 9,10-phenanthrenequinone, 1,4-benzoquinone and various other o-quinones and p-quinones (in vitro) (PubMed:19000905, PubMed:19571038, PubMed:25203508).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_032783" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207442 | CBR4 (Myc-DDK-tagged)-Human carbonyl reductase 4 (CBR4) | 10 ug |
$300.00
|
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| RC207442L3 | Lenti ORF clone of Human carbonyl reductase 4 (CBR4), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RG207442 | CBR4 (tGFP-tagged) - Human carbonyl reductase 4 (CBR4) | 10 ug |
$489.00
$500.00
|
|
| SC127775 | CBR4 (untagged)-Human carbonyl reductase 4 (CBR4) | 10 ug |
$300.00
|
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Plasmid Map for RC207442L4
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Cloning scheme for RC207442L4
Circular map for RC207442L4
Citations
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