EIF5B (NM_015904) Human Tagged Lenti ORF Clone
- LentiORF®
SKU
RC207429L4
Lenti ORF clone of Human eukaryotic translation initiation factor 5B (EIF5B), mGFP tagged
$1,362.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | EIF5B |
| Synonyms | IF2 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_015904 |
| ORF Size | 3660 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_015904.3 |
| RefSeq Size | 4698 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 9669 |
| UniProt ID | O60841 |
| Cytogenetics | 2q11.2 |
| MW | 138.8 kDa |
| Summary | Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately. provided by RefSeq, Jul 2008 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_015904" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207429 | EIF5B (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 5B (EIF5B) | 10 ug |
$589.00
$1,062.00
|
|
| RC207429L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 5B (EIF5B), Myc-DDK-tagged | 10 ug |
$1,362.00
|
|
| RG207429 | EIF5B (tGFP-tagged) - Human eukaryotic translation initiation factor 5B (EIF5B) | 10 ug |
$789.00
$1,262.00
|
|
| SC304367 | EIF5B (untagged)-Human eukaryotic translation initiation factor 5B (EIF5B) | 10 ug |
$1,169.00
|
|
Plasmid Map for RC207429L4
Download sequence for
Cloning scheme for RC207429L4
Circular map for RC207429L4
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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