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ALS2CR8 (CARF) (NM_024744) Human Tagged ORF Clone

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SKU
RC207419
ALS2CR8 (Myc-DDK-tagged)-Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $664.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ALS2CR8
Synonyms ALS2CR8; NYD-SP24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207419 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACAATCTAATGATTCATTAAGAGTCAACCATAATGACGGTGAAGAGTCAAAAACCAGTGCTCAAG
TATTTGAGCATCTAATCTGTATGGACTCCAGGGATTCTTCCTTTGGACAAAATGATTCTCCTACAGTTTT
GCCCATCACTACTCGTGAAGCAAATAATTCACTCATATCACAGAATATACCAGGGCCCCTGACTCAGACA
CAGACTCTTTCTGCAGAGCAATTCCATCTAGTGGACCAAAATGGGCAGGCTATTCAATATGAACTTCAGT
CATTGGGGGAATCCAATGCACAAATGATGATCGTTGCCAGCCCAACAGAAAATGGACAGGTACTTCGTGT
AATTCCACCTACCCAGACAGGAATGGCACAAGTGATTATACCTCAGGGGCAACTTGTGGATGTGAATAGT
CCTCGGGATGTCCCTGAAGAGAAACCCAGTAACAGAAACTTACCAACTGTAAGAGTGGATACTCTAGCAG
ACAATACCAGCAATTACATTCTTCATCCTCAAACATCCTTCCCATTGCCCAAAAAGTCAGTGACCGGAAT
GCTGGAAGAACCCCTTCTGGGGCCTCTTCAGCCACTTTCTTCTAATACACCTATATGGGCCTGCCGTCTT
AGGAGCTGTGAGAAAATTGGAGATTCATACCGTGGCTACTGTGTAAGTGAGACTGAATTAGAAAGTGTCC
TAACATTTCACAAGCAGCAAACACAGAGTGTTTGGGGGACCCGTCAGTCTCCAAGCCCAGCCAAGCCTGC
TACACGCTTGATGTGGAAATCCCAGTATGTTCCATATGATGGAATCCCATTTGTTAATGCAGGGAGTAGA
GCTGTGGTAATGGAGTGTCAGTATGGGCCAAGAAGAAAAGGTTTCCAGTTAAAAAAAGTCAGTGAGCAGG
AAAGCAGGTCTTGTCAGCTCTACAAAGCCACTTGTCCAGCTCGGATTTACATTAAAAAGGTACAGAAGTT
TCCTGAATATAGAGTTCCTACAGACCCCAAAATTGACAAGAAAATTATCAGAATGGAGCAGGAGAAAGCT
TTTAACATGCTAAAGAAGAACTTGGTAGATGCTGGTGGTGTTCTTAGGTGGTATGTACAGTTACCTACAC
AGCAAGCTCATCAGTATCATGAATTAGAGACTCCCTGCCTCACTTTGTCACCTTCTCCTTTTCCTGTGTC
TTCTCTTGAAGAAGAGGAAACTGCAGTTAGAGATGAGAATTGTGCATTACCCTCACGTTTACATCCTCAA
GTAGCACATAAGATTCAAGAATTAGTATCACAGGGAATAGAACAAGTGTATGCAGTAAGGAAACAGCTAA
GAAAATTTGTGGAAAGGGAACTGTTCAAACCCGATGAGGTACCTGAAAGACATAATTTATCTTTGTTTCC
AACTGTAAATGATATAAAAAATCACATCCATGAGGTACAGAAATCCTTGAGAAATGGAGATACGGTATAT
AACTCAGAGATTATTCCAGCAACGCTTCAATGGACTACAGACAGTGGGAATATTCTCAAAGAGACCATGA
CAGTTACATTTGCAGAAGGAAATTCACCAGGAGAATCAATTACCACCAAAGTGGAAACAAATCAGACCAG
GGGTTCTTTGTCTCCTGAGCCAACCCACTTGCTCTCCTCACTCTCCTCATTTCAGCCCAAAATATTTACA
CAACTACAGGGTTTGCAGTTACAACCAAGGTACACCTCTCCTGATGAATCACCAGCTGTGGTATCAGTAA
ATAACCAGCCGTCCTCTAGTCCTTCAGGACTTCTGGATACAATAGGAAGTGCTGTAATGAATAATAATTC
TCTACTGCTTGGTCAAAGTCATAGCCTTCAAAGAGATACATGCTTAACCCAAAACAATAGTACTGCCTCC
ACCATGGGTAACCTTCCAGAACCAGATCAAAATCTAGTTGCAATGGACGAGCTGGTAGAAGTTGGAGATG
TTGAGGATACAGGGAATCTGGAAGGAACTGTTCATCGGATTCTGTTGGGAGATGTGCAGACTATTCCAAT
ACAGATTATAGACAACCACTCAGCTCTTATTGAAGAAAATCCAGAAAGTACCATTTCTGTGAGCCAAGTT
AAACAAGAACCCAAAGAACCAGCATTGTCTATGGAAGCAAAAAAAACTGTGGACTATAAGAAATTATCTG
CTACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207419 protein sequence
Red=Cloning site Green=Tags(s)

MEQSNDSLRVNHNDGEESKTSAQVFEHLICMDSRDSSFGQNDSPTVLPITTREANNSLISQNIPGPLTQT
QTLSAEQFHLVDQNGQAIQYELQSLGESNAQMMIVASPTENGQVLRVIPPTQTGMAQVIIPQGQLVDVNS
PRDVPEEKPSNRNLPTVRVDTLADNTSNYILHPQTSFPLPKKSVTGMLEEPLLGPLQPLSSNTPIWACRL
RSCEKIGDSYRGYCVSETELESVLTFHKQQTQSVWGTRQSPSPAKPATRLMWKSQYVPYDGIPFVNAGSR
AVVMECQYGPRRKGFQLKKVSEQESRSCQLYKATCPARIYIKKVQKFPEYRVPTDPKIDKKIIRMEQEKA
FNMLKKNLVDAGGVLRWYVQLPTQQAHQYHELETPCLTLSPSPFPVSSLEEEETAVRDENCALPSRLHPQ
VAHKIQELVSQGIEQVYAVRKQLRKFVERELFKPDEVPERHNLSLFPTVNDIKNHIHEVQKSLRNGDTVY
NSEIIPATLQWTTDSGNILKETMTVTFAEGNSPGESITTKVETNQTRGSLSPEPTHLLSSLSSFQPKIFT
QLQGLQLQPRYTSPDESPAVVSVNNQPSSSPSGLLDTIGSAVMNNNSLLLGQSHSLQRDTCLTQNNSTAS
TMGNLPEPDQNLVAMDELVEVGDVEDTGNLEGTVHRILLGDVQTIPIQIIDNHSALIEENPESTISVSQV
KQEPKEPALSMEAKKTVDYKKLSAT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024744
ORF Size 2175 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024744.17
RefSeq Size 6253 bp
RefSeq ORF 2178 bp
Locus ID 79800
UniProt ID Q8N187
Cytogenetics 2q33.2
MW 80.7 kDa
Summary Acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024744" in other vectors (6)
SKU Description Size Price
RC207419L3 Lenti ORF clone of Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8), Myc-DDK-tagged 10 ug
$964.00
RC207419L4 Lenti ORF clone of Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8), mGFP tagged 10 ug
$964.00
RG207419 ALS2CR8 (tGFP-tagged) - Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8) 10 ug
$864.00
SC112033 ALS2CR8 (untagged)-Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8) 10 ug
$658.00
SC318208 ALS2CR8 (untagged)-Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8) 10 ug
$731.00
SC323963 ALS2CR8 (untagged)-Human amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8 (ALS2CR8) 10 ug
$665.00

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