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GEFT (ARHGEF25) (NM_182947) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC207415L4
Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $840.00
3 Weeks*
Size
Bulk Requests & Clone Modifications

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Other products for "GEFT or NM_182947"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GEFT
Synonyms GEFT; p63RhoGEF
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207415).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182947
ORF Size 1740 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182947.1, NP_891992.1
RefSeq Size 2563 bp
RefSeq ORF 1743 bp
Locus ID 115557
UniProt ID Q86VW2
Cytogenetics 12q13.3
MW 64 kDa
Summary Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2012
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Documents
Resources
"NM_182947" in other vectors (6)
SKU Description Size Price
RC207415 ARHGEF25 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1 10 ug
$540.00
RC207415L1 Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, Myc-DDK-tagged 10 ug
$840.00
RC207415L2 Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, mGFP tagged 10 ug
$840.00
RC207415L3 Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, Myc-DDK-tagged 10 ug
$840.00
RG207415 ARHGEF25 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1 10 ug
$489.00 $740.00
SC307541 ARHGEF25 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1 10 ug
$595.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.