DCP1B (NM_152640) Human Tagged ORF Clone

CAT#: RC207398

DCP1B (Myc-DDK-tagged)-Human DCP1 decapping enzyme homolog B (S. cerevisiae) (DCP1B)

Product Images

Circular map for RC207398

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY DCP1B (Cat# RC207398, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCP1B (Cat# TA809933)(1:2000). Positive lysates LY407370 (100ug) and LC407370 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY407370) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207398 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified DCP1B protein (Cat# TP307398). The protein was produced from HEK293T cells transfected with DCP1B cDNA clone (Cat# RC207398) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCP1B
• Synonyms: DCP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207398 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCGTGGCGGCAGGCGGCCTGGTGGGAAAGGGGCGCGACATCAGCCTAGCGGCCCTGCAGCGCC
  ACGACCCCTATATCAACCGCATCGTGGACGTGGCCAGCCAGGTGGCTCTGTACACCTTCGGCCATCGGGC
  CAACGAGTGGGAGAAAACTGATGTGGAAGGAACCTTATTTGTTTATACAAGGTCTGCTTCTCCAAAGCAT
  GGATTCACCATTATGAATAGGCTGAGCATGGAAAATAGGACAGAACCTATTACTAAAGACTTGGATTTCC
  AACTCCAGGACCCTTTCCTTCTCTACAGAAATGCCAGATTGTCCATCTATGGAATTTGGTTTTATGATAA
  GGAAGAATGCCAAAGAATTGCAGAGCTTATGAAAAACCTAACTCAGTATGAACAGTTGAAAGCCCATCAG
  GGAACTGGAGCAGGAATTTCACCAGTGATCCTCAATTCAGGAGAGGGCAAAGAAGTAGACATTTTACGAA
  TGCTCATCAAGGCCAAAGACGAATACACAAAGTGTAAAACCTGTTCTGAGCCAAAAAAGATAACCAGTTC
  CTCTGCCATCTATGACAATCCAAATCTCATCAAACCAATTCCAGTGAAACCCAGTGAAAACCAGCAACAG
  CGTATACCTCAGCCCAACCAGACCTTAGACCCTGAACCCCAACACTTATCCTTGACAGCTCTGTTTGGGA
  AGCAGGACAAAGCTACATGTCAGGAAACTGTGGAGCCTCCGCAGACTCTCCACCAGCAGCAGCAGCAGCA
  GCAGCAGCAGCAGCAAGAGAAGCTTCCAATTAGGCAGGGGGTTGTACGCTCCCTGTCCTATGAGGAACCC
  AGAAGACACTCACCCCCCATTGAGAAGCAGCTCTGTCCAGCCATTCAGAAACTCATGGTCAGGAGCGCAG
  ACCTCCACCCATTGTCAGAGCTGCCTGAAAACCGGCCTTGTGAAAATGGCAGTACCCATTCTGCGGGAGA
  ATTTTTTACAGGACCTGTCCAGCCAGGGTCTCCTCACAACATTGGAACTTCTCGTGGTGTACAAAATGCT
  TCCAGAACTCAGAACCTGTTCGAGAAACTTCAGAGTACCCCAGGGGCAGCAAACAAGTGTGACCCTAGTA
  CACCAGCACCTGCCAGCTCAGCTGCCCTGAACCGCAGCAGAGCTCCCACTTCTGTCACCCCTGTGGCTCC
  AGGAAAGGGTCTGGCTCAGCCACCACAGGCCTATTTCAATGGCTCCCTTCCACCTCAGACAGTAGGACAT
  CAGGCTCATGGAAGAGAACAGTCCACACTCCCAAGACAAACACTCGCCATCTCTGGTAGTCAGACTGGCA
  GCTCTGGAGTGATCTCCCCTCAAGAGTTACTGAAGAAGCTTCAGATTGTACAGCAGGAGCAGCAGCTGCA
  TGCCTCTAACCGGCCAGCCTTGGCCGCTAAGTTTCCTGTGCTCGCTCAGAGCTCTGGAACAGGGAAACCC
  TTGGAATCCTGGATCAACAAGACACCCAACACAGAACAGCAGACTCCTCTTTTCCAGGTCATCTCACCTC
  AGCGCATCCCTGCTACAGCAGCTCCGTCTCTGCTTATGTCCCCCATGGTGTTCGCACAACCCACCTCCGT
  CCCGCCAAAGGAAAGGGAGAGCGGCCTCTTGCCTGTGGGAGGCCAGGAGCCACCTGCTGCTGCCACCAGC
  CTCCTCCTGCCCATACAGAGCCCGGAGCCCTCCGTGATCACCAGCAGCCCACTCACCAAGCTCCAGCTCC
  AGGAGGCACTGCTGTACCTCATTCAGAATGATGACAACTTCTTAAATATAATCTATGAAGCCTATCTCTT
  CAGCATGACTCAAGCAGCCATGAAAAAGACTATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207398 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAVAAGGLVGKGRDISLAALQRHDPYINRIVDVASQVALYTFGHRANEWEKTDVEGTLFVYTRSASPKH
  GFTIMNRLSMENRTEPITKDLDFQLQDPFLLYRNARLSIYGIWFYDKEECQRIAELMKNLTQYEQLKAHQ
  GTGAGISPVILNSGEGKEVDILRMLIKAKDEYTKCKTCSEPKKITSSSAIYDNPNLIKPIPVKPSENQQQ
  RIPQPNQTLDPEPQHLSLTALFGKQDKATCQETVEPPQTLHQQQQQQQQQQQEKLPIRQGVVRSLSYEEP
  RRHSPPIEKQLCPAIQKLMVRSADLHPLSELPENRPCENGSTHSAGEFFTGPVQPGSPHNIGTSRGVQNA
  SRTQNLFEKLQSTPGAANKCDPSTPAPASSAALNRSRAPTSVTPVAPGKGLAQPPQAYFNGSLPPQTVGH
  QAHGREQSTLPRQTLAISGSQTGSSGVISPQELLKKLQIVQQEQQLHASNRPALAAKFPVLAQSSGTGKP
  LESWINKTPNTEQQTPLFQVISPQRIPATAAPSLLMSPMVFAQPTSVPPKERESGLLPVGGQEPPAAATS
  LLLPIQSPEPSVITSSPLTKLQLQEALLYLIQNDDNFLNIIYEAYLFSMTQAAMKKTM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152640
• ORF Size: 1854 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152640.5
• RefSeq Size: 2105 bp
• RefSeq ORF: 1854 bp
• Locus ID: 196513
• UniProt ID: Q8IZD4
• Cytogenetics: 12p13.33
• Protein Pathways: RNA degradation
• MW: 67.8 kDa
• Gene Summary: This gene encodes a member of a family of proteins that function in removing the 5' cap from mRNAs, which is a step in regulated mRNA decay. This protein localizes to cytoplasmic foci which are the site of mRNA breakdown and turnover. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]