TMEM65 (NM_194291) Human Tagged ORF Clone

CAT#: RC207368

TMEM65 (Myc-DDK-tagged)-Human transmembrane protein 65 (TMEM65)

Product Images

Western blot validation of overexpression lysate (Cat# LY405125) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207368 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207368 representing NM_194291
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGCTGCTGCGGCTGCTGAGGAGCCGGACCGCGCGCAGCCTGAGGCCGGGCCCGGCCGCCGCCG
  CCGCGCCCCGCCCGCCGTCCTGGTGCTGCTGCGGGCGGGGGCTGCTGGCGCTCGCGCCCCCCGGCGGCTT
  GCCGGGCGGCCCCAGGCGGCTGGGCACGCACCCCAAGAAGGAGCCCATGGAGGCGCTGAACACGGCGCAG
  GGCGCGCGCGACTTCATCTACAGCCTGCACTCCACGGAGAGGAGCTGCCTGCTCAAAGAGCTGCACCGCT
  TCGAGTCTGTTGCCATTGCCCAAGAAAAATTGGAAGCTCCACCACCCACCCCAGGACAGCTGAGATATGT
  ATTCATCCACAATGCGATACCTTTCATAGGGTTTGGCTTTTTGGATAATGCAATTATGATTGTTGCTGGA
  ACCCATATTGAAATGTCTATTGGAATTATTTTGGGAATTTCAACTATGGCAGCTGCTGCTTTGGGAAATC
  TTGTGTCAGATCTAGCTGGACTTGGACTTGCAGGCTACGTTGAAGCATTGGCTTCCAGGTTAGGCCTGTC
  AATTCCTGATCTCACACCAAAGCAAGTTGACATGTGGCAAACACGTCTTAGTACACATTTGGGCAAAGCT
  GTTGGGGTGACTATTGGCTGCATTCTAGGAATGTTTCCTTTAATTTTCTTTGGAGGAGGTGAAGAAGATG
  AAAAACTGGAAACGAAAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207368 representing NM_194291
  Red=Cloning site Green=Tags(s)
  
  MSRLLRLLRSRTARSLRPGPAAAAAPRPPSWCCCGRGLLALAPPGGLPGGPRRLGTHPKKEPMEALNTAQ
  GARDFIYSLHSTERSCLLKELHRFESVAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAG
  THIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKA
  VGVTIGCILGMFPLIFFGGGEEDEKLETKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194291
• ORF Size: 720 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194291.1, NP_919267.1
• RefSeq Size: 3258 bp
• RefSeq ORF: 723 bp
• Locus ID: 157378
• UniProt ID: Q6PI78
• Cytogenetics: 8q24.13
• Protein Families: Transmembrane
• MW: 25.3 kDa
• Gene Summary: May play an important role in cardiac development and function. May regulate cardiac conduction and the function of the gap junction protein GJA1. May contributes to the stability and proper localization of GJA1 to cardiac intercalated disk thereby regulating gap junction communication (By similarity). May also play a role in the regulation of mitochondrial respiration and mitochondrial DNA copy number maintenance (PubMed:28295037).[UniProtKB/Swiss-Prot Function]