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ANKRD15 (KANK1) (NM_015158) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC207331L1
Lenti ORF clone of Human KN motif and ankyrin repeat domains 1 (KANK1), transcript variant 1, Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,456.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ANKRD15
Synonyms ANKRD15; CPSQ2; KANK
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207331).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015158
ORF Size 4056 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015158.2
RefSeq Size 5083 bp
RefSeq ORF 4059 bp
Locus ID 23189
UniProt ID Q14678
Cytogenetics 9p24.3
MW 147.1 kDa
Summary The protein encoded by this gene belongs to the Kank family of proteins, which contain multiple ankyrin repeat domains. This family member functions in cytoskeleton formation by regulating actin polymerization. This gene is a candidate tumor suppressor for renal cell carcinoma. Mutations in this gene cause cerebral palsy spastic quadriplegic type 2, a central nervous system development disorder. A t(5;9) translocation results in fusion of the platelet-derived growth factor receptor beta gene (PDGFRB) on chromosome 5 with this gene in a myeloproliferative neoplasm featuring severe thrombocythemia. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 20. provided by RefSeq, Dec 2014
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Documents
Resources
"NM_015158" in other vectors (6)
SKU Description Size Price
RC207331 KANK1 (Myc-DDK-tagged)-Human KN motif and ankyrin repeat domains 1 (KANK1), transcript variant 1 10 ug
$1,156.00
RC207331L2 Lenti ORF clone of Human KN motif and ankyrin repeat domains 1 (KANK1), transcript variant 1, mGFP tagged 10 ug
$1,456.00
RC207331L3 Lenti ORF clone of Human KN motif and ankyrin repeat domains 1 (KANK1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,456.00
RC207331L4 Lenti ORF clone of Human KN motif and ankyrin repeat domains 1 (KANK1), transcript variant 1, mGFP tagged 10 ug
$1,456.00
RG207331 KANK1 (tGFP-tagged) - Human KN motif and ankyrin repeat domains 1 (KANK1), transcript variant 1 10 ug
$1,356.00
SC309139 KANK1 (untagged)-Human KN motif and ankyrin repeat domains 1 (KANK1), transcript variant 1 10 ug
$1,273.00

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