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LOC158381 (NM_001029857) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC207277
LOC158381 (Myc-DDK-tagged)-Human hypothetical protein LOC158381 (LOC158381)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LOC158381
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207277 representing NM_001029857
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACAGATGGCAGTGTCCCAGAAGCCCAGTCAACATTAGACTCTACATCTTCATCTATGCAGCCAAGC
CCTGTATCAAATCAGTCACTTTTATCAGAATCTGTAGCATCTTCTCAATTGGACAGTACATCTGTGGACA
AGCAGTACCTGAAACAGAAGATCTGCAGGCTTCAGTATCAGACATGGCACAGCAGCCATCTGAAGAGCAA
AGCAAGTCTCTTGAAAAACCAAAACAAAAAAAGAATCTCTGTTTCATGTGCAGGAAGAAAGTGGGACTTA
CTAGGTTTGAATGCGGTGTAGAAATGTTTACTGTGGTGTACACTGTTACTCAGATCTACACAATTGCTCT
TAAAATTACAAAGCTGATGCCGCTGAGAAAATCAGAAAAGAAAATCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC207277 representing NM_001029857
Red=Cloning site Green=Tags(s)

MHRWQCPRSPVNIRLYIFIYAAKPCIKSVTFIRICSIFSIGQYICGQAVPETEDLQASVSDMAQQPSEEQ
SKSLEKPKQKKNLCFMCRKKVGLTRFECGVEMFTVVYTVTQIYTIALKITKLMPLRKSEKKIQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001029857
ORF Size 399 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001029857.2, NP_001025028.2
RefSeq Size 3980 bp
RefSeq ORF 401 bp
Locus ID 158381
Cytogenetics 9p13.3
MW 15.8 kDa
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Documents
Resources
"NM_001029857" in other vectors (3)
SKU Description Size Price
RC207277L3 Lenti ORF clone of Human hypothetical protein LOC158381 (LOC158381), Myc-DDK-tagged 10 ug
$450.00
RC207277L4 Lenti ORF clone of Human hypothetical protein LOC158381 (LOC158381), mGFP tagged 10 ug
$450.00
RG207277 LOC158381 (tGFP-tagged) - Human hypothetical protein LOC158381 (LOC158381) 10 ug
$489.00

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