GCLM (NM_002061) Human Tagged ORF Clone
GCLM (Myc-DDK-tagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM)
|Type||Human Tagged ORF Clone|
|E. coli Selection||Kanamycin (25 ug/mL)|
>RC207224 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)
>RC207224 protein sequence
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||822 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||3074 bp|
|RefSeq ORF||825 bp|
|Protein Families||Druggable Genome|
|Protein Pathways||Glutathione metabolism, Metabolic pathways|
|Gene Summary||Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]|
|cDNA Clone Resources|
|RC207224L1||Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), Myc-DDK-tagged||
|RC207224L2||Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), mGFP tagged||
|RC207224L3||Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), Myc-DDK-tagged||
|RC207224L4||Lenti ORF clone of Human glutamate-cysteine ligase, modifier subunit (GCLM), mGFP tagged||
|RG207224||GCLM (GFP-tagged) - Human glutamate-cysteine ligase, modifier subunit (GCLM)||
|SC118877||GCLM (untagged)-Human glutamate-cysteine ligase, modifier subunit (GCLM)||