PARN (NM_002582) Human Tagged ORF Clone

CAT#: RC207220

PARN (Myc-DDK-tagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1

Product Images

Circular map for RC207220

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PARN (Cat# RC207220, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PARN(Cat# TA802668). Positive lysates LY419233 (100ug) and LC419233 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY419233) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207220 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PARN protein (Cat# TP307220). The protein was produced from HEK293T cells transfected with PARN cDNA clone (Cat# RC207220) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PARN
• Synonyms: DAN; DKCB6; PFBMFT4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207220 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCCGACT
  TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTT
  TGACACTCCAGAAGAGAGGTATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTATTTCAGTTTGGC
  CTTTGCACTTTTAAGTATGACTACACAGATTCAAAGTATATAACGAAGTCATTTAACTTCTATGTTTTCC
  CGAAACCCTTCAATAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTAGC
  AAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAAGA
  CAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTATGTATCTC
  CTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAGAA
  AATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAAGA
  AAACTAATTTATCAGACTTTGAGCTGGAAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTGAAA
  AGAAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAACA
  TGCCAAAGAACAGGAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATTCG
  GGAAAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTACTGCCCTC
  TGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGACTCTTGGATACTAAATT
  GATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACATCCCTTGCGGAATTGGAAAAGCGGTTA
  AAAGAGACACCTTTCAACCCTCCTAAAGTTGAAAGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTCTG
  AACAACTCCACGAGGCAGGCTACGATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTACCT
  AGGTTCTTTTCTCAGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTAAC
  AAGTTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAGCCTAAAC
  GTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTTTACCAGCTTTTCAGTGC
  CTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCATTTGTTTCCCTTAGCCAGCCCGAGCAA
  GTAAAGATTGCTGTCAATACCAGCAAATATGCAGAAAGCTATCGGATCCAAACCTATGCTGAATATATGG
  GGAGAAAACAGGAAGAGAAGCAGATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGCAA
  ACGGTTAAACCCCCAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAGCT
  CCCAGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGAGTGTCAG
  GGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTCTCAGAGGGAAGGAAAAA
  GGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCAGGAAGCATCTCGAAGAACAGCCCTGCC
  ACACTCTTTGAAGTTCCTGACACATGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207220 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFG
  LCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEER
  QLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQR
  KLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANS
  GKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRL
  KETPFNPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSFLSPPKIHVSARSKLIEPFFN
  KLFLMRVMDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQ
  VKIAVNTSKYAESYRIQTYAEYMGRKQEEKQIKRKWTEDSWKEADSKRLNPQCIPYTLQNHYYRNNSFTA
  PSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEPLSEGRKKAKKLKRMKKELSPAGSISKNSPA
  TLFEVPDTW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002582
• ORF Size: 1917 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002582.4
• RefSeq Size: 3083 bp
• RefSeq ORF: 1920 bp
• Locus ID: 5073
• UniProt ID: O95453
• Cytogenetics: 16p13.12
• Domains: R3H, CAF1
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• MW: 73.5 kDa
• Gene Summary: The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]