PARN (NM_002582) Human Tagged ORF Clone

CAT#: RC207220

Reviews ()
Write a review

PARN (Myc-DDK-tagged)-Human poly(A)-specific ribonuclease (deadenylation nuclease) (PARN), transcript variant 1



  "NM_002582" in other vectors (6)

Reconstitution Protocol

USD 595.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 177.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 650.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 160.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 59.00

Other products for "PARN"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PARN
Synonyms DAN; DKCB6; PFBMFT4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC207220 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATAATCAGGAGCAATTTTAAGAGTAATCTTCACAAAGTGTACCAGGCCATAGAGGAGGCCGACT
TCTTCGCCATCGATGGGGAGTTTTCAGGAATCAGTGATGGACCTTCAGTCTCTGCATTAACAAATGGTTT
TGACACTCCAGAAGAGAGGTATCAGAAGCTTAAAAAGCATTCCATGGACTTTTTGCTATTTCAGTTTGGC
CTTTGCACTTTTAAGTATGACTACACAGATTCAAAGTATATAACGAAGTCATTTAACTTCTATGTTTTCC
CGAAACCCTTCAATAGATCCTCACCAGATGTCAAATTTGTTTGTCAGAGCTCCAGCATTGACTTTCTAGC
AAGCCAGGGATTTGATTTTAATAAAGTTTTTCGAAATGGAATTCCATATTTAAATCAGGAAGAAGAAAGA
CAGTTAAGAGAGCAGTATGATGAAAAACGTTCACAGGCGAATGGTGCAGGAGCTCTGTCCTATGTATCTC
CTAACACTTCAAAATGTCCTGTCACGATTCCTGAGGATCAAAAGAAGTTTATTGACCAAGTGGTAGAGAA
AATAGAGGATTTATTACAAAGTGAAGAAAACAAGAACTTGGATTTAGAGCCATGTACCGGGTTCCAAAGA
AAACTAATTTATCAGACTTTGAGCTGGAAGTATCCGAAAGGCATTCATGTTGAGACTTTAGAAACTGAAA
AGAAGGAGCGATATATAGTTATCAGCAAAGTAGATGAAGAAGAACGCAAAAGAAGAGAGCAGCAGAAACA
TGCCAAAGAACAGGAGGAGCTGAATGATGCTGTGGGATTTTCTAGAGTCATTCACGCCATTGCTAATTCG
GGAAAACTTGTTATTGGACACAATATGCTCTTGGACGTCATGCACACAGTTCATCAGTTCTACTGCCCTC
TGCCTGCGGACTTAAGTGAGTTTAAAGAGATGACAACATGTGTTTTCCCCAGACTCTTGGATACTAAATT
GATGGCCAGCACACAACCTTTTAAGGATATCATTAACAACACATCCCTTGCGGAATTGGAAAAGCGGTTA
AAAGAGACACCTTTCAACCCTCCTAAAGTTGAAAGTGCCGAAGGTTTTCCAAGTTATGACACAGCCTCTG
AACAACTCCACGAGGCAGGCTACGATGCCTACATCACAGGGCTGTGCTTCATCTCCATGGCCAATTACCT
AGGTTCTTTTCTCAGCCCTCCAAAAATTCATGTGTCTGCCAGATCAAAACTCATTGAACCTTTTTTTAAC
AAGTTATTTCTTATGAGGGTCATGGATATCCCCTATCTAAACTTGGAAGGACCAGACTTGCAGCCTAAAC
GTGATCATGTTCTCCATGTGACATTCCCCAAAGAATGGAAAACCAGCGACCTTTACCAGCTTTTCAGTGC
CTTTGGTAACATTCAGATATCCTGGATTGATGACACATCAGCATTTGTTTCCCTTAGCCAGCCCGAGCAA
GTAAAGATTGCTGTCAATACCAGCAAATATGCAGAAAGCTATCGGATCCAAACCTATGCTGAATATATGG
GGAGAAAACAGGAAGAGAAGCAGATCAAAAGAAAGTGGACTGAAGATAGCTGGAAGGAGGCTGACAGCAA
ACGGTTAAACCCCCAGTGCATACCCTACACCCTGCAGAATCACTATTACCGCAACAATAGTTTTACAGCT
CCCAGCACAGTAGGAAAGAGAAATTTGAGTCCTAGTCAAGAGGAAGCTGGCCTGGAGGACGGAGTGTCAG
GGGAGATTTCCGACACTGAGCTTGAGCAGACCGATTCCTGTGCAGAGCCCCTCTCAGAGGGAAGGAAAAA
GGCCAAGAAATTAAAAAGAATGAAGAAGGAGCTTTCTCCAGCAGGAAGCATCTCGAAGAACAGCCCTGCC
ACACTCTTTGAAGTTCCTGACACATGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC207220 protein sequence
Red=Cloning site Green=Tags(s)

MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTPEERYQKLKKHSMDFLLFQFG
LCTFKYDYTDSKYITKSFNFYVFPKPFNRSSPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEER
QLREQYDEKRSQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNLDLEPCTGFQR
KLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQEELNDAVGFSRVIHAIANS
GKLVIGHNMLLDVMHTVHQFYCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRL
KETPFNPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSFLSPPKIHVSARSKLIEPFFN
KLFLMRVMDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQ
VKIAVNTSKYAESYRIQTYAEYMGRKQEEKQIKRKWTEDSWKEADSKRLNPQCIPYTLQNHYYRNNSFTA
PSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEPLSEGRKKAKKLKRMKKELSPAGSISKNSPA
TLFEVPDTW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002582
ORF Size 1917 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002582.4
RefSeq Size 3083 bp
RefSeq ORF 1920 bp
Locus ID 5073
UniProt ID O95453
Cytogenetics 16p13.12
Domains R3H, CAF1
Protein Families Transcription Factors
Protein Pathways RNA degradation
MW 73.5 kDa
Gene Summary The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Other Versions

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.