PTK9 (TWF1) (NM_002822) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC207215L4
Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), mGFP tagged
  $757.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PTK9
Synonyms A6; PTK9
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207215).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002822
ORF Size 1152 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002822.3, NP_002813.2
RefSeq Size 3062 bp
RefSeq ORF 1053 bp
Locus ID 5756
UniProt ID Q12792
Cytogenetics 12q12
Domains ADF
Protein Families Druggable Genome, Protein Kinase
MW 43.9 kDa
Summary This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002822" in other vectors (8)
SKU Description Size Price
RC207215 TWF1 (Myc-DDK-tagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) 10 ug
$457.00
RC207215L1 Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), Myc-DDK-tagged 10 ug
$757.00
RC207215L2 Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), mGFP tagged 10 ug
$757.00
RC207215L3 Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), Myc-DDK-tagged 10 ug
$757.00
RG207215 TWF1 (tGFP-tagged) - Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) 10 ug
$489.00 $657.00
SC109662 TWF1 (untagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) 10 ug
$457.00
SC317613 TWF1 (untagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) 10 ug
$457.00
SC317614 TWF1 (untagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) 10 ug
$503.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.