AKAP3 (NM_006422) Human Tagged ORF Clone
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | AKAP3 |
| Synonyms | AKAP 110; AKAP110; CT82; FSP95; HEL159; PRKA3; SOB1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
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Plasmid Map
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| ACCN | NM_006422 |
| ORF Size | 2559 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_006422.3 |
| RefSeq Size | 3323 bp |
| RefSeq ORF | 2562 bp |
| Locus ID | 10566 |
| UniProt ID | O75969 |
| Cytogenetics | 12p13.32 |
| Protein Families | Druggable Genome |
| MW | 94.8 kDa |
| Summary | This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction. provided by RefSeq, May 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_006422" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207199L3 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 3 (AKAP3), Myc-DDK-tagged | 10 ug |
$1,081.00
|
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| RC207199L4 | Lenti ORF clone of Human A kinase (PRKA) anchor protein 3 (AKAP3), mGFP tagged | 10 ug |
$1,081.00
|
|
| RG207199 | AKAP3 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 3 (AKAP3) | 10 ug |
$789.00
$981.00
|
|
| SC126490 | AKAP3 (untagged)-Human A kinase (PRKA) anchor protein 3 (AKAP3) | 10 ug |
$782.00
|
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Plasmid Map for RC207199
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Cloning scheme for RC207199
Circular map for RC207199
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