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Muscarinic Acetylcholine Receptor M5 (CHRM5) (NM_012125) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC207196L1
Lenti ORF clone of Human cholinergic receptor, muscarinic 5 (CHRM5), Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $795.00
In Stock*
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Bulk Requests & Clone Modifications

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Other products for "Muscarinic Acetylcholine Receptor M5 or NM_012125"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Muscarinic Acetylcholine Receptor M5
Synonyms HM5
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC207196).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012125
ORF Size 1597 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012125.2
RefSeq Size 3055 bp
RefSeq ORF 1599 bp
Locus ID 1133
UniProt ID P08912
Cytogenetics 15q14
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
MW 60.1 kDa
Summary The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. provided by RefSeq, Jul 2008
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Documents
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"NM_012125" in other vectors (6)
SKU Description Size Price
RC207196 CHRM5 (Myc-DDK-tagged)-Human cholinergic receptor, muscarinic 5 (CHRM5) 10 ug
$495.00
RC207196L2 Lenti ORF clone of Human cholinergic receptor, muscarinic 5 (CHRM5), mGFP tagged 10 ug
$795.00
RC207196L3 Lenti ORF clone of Human cholinergic receptor, muscarinic 5 (CHRM5), Myc-DDK-tagged 10 ug
$795.00
RC207196L4 Lenti ORF clone of Human cholinergic receptor, muscarinic 5 (CHRM5), mGFP tagged 10 ug
$795.00
RG207196 CHRM5 (tGFP-tagged) - Human cholinergic receptor, muscarinic 5 (CHRM5) 10 ug
$489.00 $695.00
SC115522 CHRM5 (untagged)-Human cholinergic receptor, muscarinic 5 (CHRM5) 10 ug
$496.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.