YTHDF1 (NM_017798) Human Tagged ORF Clone

CAT#: RC207185

YTHDF1 (Myc-DDK-tagged)-Human YTH domain family, member 1 (YTHDF1)

Product Images

Circular map for RC207185

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  Western blot validation of overexpression lysate (Cat# LY413537) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207185 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified YTHDF1 protein (Cat# TP307185). The protein was produced from HEK293T cells transfected with YTHDF1 cDNA clone (Cat# RC207185) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: YTHDF1
• Synonyms: C20orf21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207185 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCCACCAGCGTGGACACCCAGAGAACAAAAGGACAAGATAATAAAGTACAAAATGGTTCGTTAC
  ATCAGAAGGATACAGTTCATGACAATGACTTTGAGCCCTACCTTACTGGACAGTCAAATCAGAGTAACAG
  TTACCCCTCAATGAGCGACCCCTACCTGTCCAGCTATTACCCGCCGTCCATTGGATTTCCTTACTCCCTC
  AATGAGGCTCCGTGGTCTACTGCAGGGGACCCTCCGATTCCATACCTCACCACCTACGGACAGCTCAGTA
  ACGGAGACCATCATTTTATGCACGATGCTGTTTTTGGGCAGCCTGGGGGCCTGGGGAACAACATCTATCA
  GCACAGGTTCAATTTTTTCCCTGAAAACCCTGCGTTCTCAGCATGGGGGACAAGTGGGTCTCAAGGTCAG
  CAGACCCAGAGCTCCGCGTATGGGAGCAGCTACACCTACCCCCCGAGCTCCCTGGGTGGCACGGTGGTTG
  ATGGGCAGCCAGGCTTTCACAGCGACACCCTCAGCAAGGCCCCCGGGATGAACAGCCTGGAGCAGGGCAT
  GGTTGGCCTGAAGATTGGGGACGTCAGCTCCTCCGCCGTCAAGACGGTGGGCTCTGTCGTCAGCAGCGTG
  GCACTGACTGGTGTCCTTTCTGGCAACGGTGGGACAAATGTGAACATGCCAGTTTCAAAGCCGACCTCGT
  GGGCTGCCATTGCCAGCAAGCCTGCAAAACCACAGCCTAAAATGAAAACAAAGAGCGGGCCTGTCATGGG
  GGGTGGGCTGCCCCCTCCACCCATAAAGCATAACATGGACATTGGCACCTGGGATAACAAGGGGCCTGTG
  CCGAAGGCCCCAGTCCCCCAGCAGGCACCCTCTCCACAGGCTGCCCCACAGCCCCAGCAGGTGGCTCAGC
  CTCTCCCAGCACAGCCCCCAGCTTTGGCTCAACCGCAGTATCAGAGCCCTCAGCAGCCACCCCAGACCCG
  CTGGGTTGCCCCACGCAACAGAAACGCGGCGTTTGGGCAGAGCGGAGGGGCTGGCAGCGATAGCAACTCT
  CCTGGAAACGTCCAGCCTAATTCTGCCCCCAGCGTCGAATCCCACCCCGTCCTTGAAAAACTGAAGGCTG
  CTCACAGCTACAACCCGAAAGAGTTTGAGTGGAATCTGAAAAGCGGGCGTGTGTTCATCATCAAGAGCTA
  CTCTGAGGACGACATCCACCGCTCCATTAAGTACTCCATCTGGTGTAGCACAGAGCACGGCAACAAGCGC
  CTGGACAGCGCCTTCCGCTGCATGAGCAGCAAGGGGCCCGTCTACCTGCTCTTCAGCGTCAATGGGAGTG
  GGCATTTTTGTGGGGTGGCCGAGATGAAGTCCCCCGTGGACTACGGCACCAGTGCCGGGGTCTGGTCTCA
  GGACAAGTGGAAGGGGAAGTTTGATGTCCAGTGGATTTTTGTTAAGGATGTACCCAATAACCAGCTCCGG
  CACATCAGGCTGGAGAATAACGACAACAAACCGGTCACAAACTCCCGGGACACCCAGGAGGTGCCCTTAG
  AAAAAGCCAAGCAAGTGCTGAAAATTATCAGTTCCTACAAGCACACAACCTCCATCTTCGACGACTTTGC
  TCACTACGAGAAGCGCCAGGAGGAGGAGGAGGTGGTGCGCAAGGAACGGCAGAGTCGAAACAAACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207185 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSATSVDTQRTKGQDNKVQNGSLHQKDTVHDNDFEPYLTGQSNQSNSYPSMSDPYLSSYYPPSIGFPYSL
  NEAPWSTAGDPPIPYLTTYGQLSNGDHHFMHDAVFGQPGGLGNNIYQHRFNFFPENPAFSAWGTSGSQGQ
  QTQSSAYGSSYTYPPSSLGGTVVDGQPGFHSDTLSKAPGMNSLEQGMVGLKIGDVSSSAVKTVGSVVSSV
  ALTGVLSGNGGTNVNMPVSKPTSWAAIASKPAKPQPKMKTKSGPVMGGGLPPPPIKHNMDIGTWDNKGPV
  PKAPVPQQAPSPQAAPQPQQVAQPLPAQPPALAQPQYQSPQQPPQTRWVAPRNRNAAFGQSGGAGSDSNS
  PGNVQPNSAPSVESHPVLEKLKAAHSYNPKEFEWNLKSGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKR
  LDSAFRCMSSKGPVYLLFSVNGSGHFCGVAEMKSPVDYGTSAGVWSQDKWKGKFDVQWIFVKDVPNNQLR
  HIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIISSYKHTTSIFDDFAHYEKRQEEEEVVRKERQSRNKQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017798
• ORF Size: 1677 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017798.4
• RefSeq Size: 3277 bp
• RefSeq ORF: 1680 bp
• Locus ID: 54915
• UniProt ID: Q9BYJ9
• Cytogenetics: 20q13.33
• MW: 60.9 kDa
• Gene Summary: Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory (By similarity). Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation (By similarity). Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells (By similarity). Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells (By similarity). In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation (By similarity).[UniProtKB/Swiss-Prot Function]