Mitofusin 1 (MFN1) (NM_033540) Human Tagged ORF Clone

CAT#: RC207184

MFN1 (Myc-DDK-tagged)-Human mitofusin 1 (MFN1), nuclear gene encoding mitochondrial protein

Product Images

Circular map for RC207184

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MFN1 (Cat# RC207184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MFN1 (Cat# TA802148). Positive lysates LY403252 (100ug) and LC403252 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403252) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207184 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MFN1 protein (Cat# TP307184). The protein was produced from HEK293T cells transfected with MFN1 cDNA clone (Cat# RC207184) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mitofusin 1
• Synonyms: hfzo1; hfzo2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207184 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAACCTGTTTCTCCACTGAAGCACTTTGTGCTGGCTAAGAAGGCGATTACTGCAATCTTTGACC
  AGTTACTGGAGTTTGTTACTGAAGGATCACATTTTGTTGAAGCAACATATAAGAATCCGGAACTTGATCG
  AATAGCCACTGAAGATGATCTGGTAGAAATGCAAGGATATAAAGACAAGCTTTCCATCATTGGTGAGGTG
  CTATCTCGGAGACACATGAAGGTGGCATTTTTTGGCAGGACAAGCAGTGGGAAGAGCTCTGTTATCAATG
  CAATGTTGTGGGATAAAGTTCTCCCTAGTGGGATTGGCCATATAACCAATTGCTTCCTAAGTGTTGAAGG
  AACTGATGGAGATAAAGCCTATCTTATGACAGAAGGATCAGATGAAAAAAAGAGTGTGAAGACAGTTAAT
  CAACTGGCCCATGCCCTTCACATGGACAAAGATTTGAAAGCTGGCTGTCTTGTACGTGTGTTTTGGCCAA
  AAGCAAAATGTGCCCTCTTGAGAGATGACCTGGTGTTAGTAGACAGTCCAGGCACAGATGTCACTACAGA
  GCTGGATAGCTGGATTGATAAGTTTTGCCTAGATGCTGATGTCTTTGTTTTGGTCGCAAACTCTGAATCA
  ACACTAATGAATACGGAAAAACACTTTTTTCACAAGGTGAATGAGCGGCTTTCCAAGCCTAATATTTTCA
  TTCTCAATAATCGTTGGGATGCCTCTGCATCAGAGCCAGAATATATGGAAGACGTACGCAGACAGCACAT
  GGAAAGATGCCTGCATTTCTTGGTGGAGGAGCTCAAAGTTGTAAATGCTTTAGAAGCACAGAATCGTATC
  TTCTTTGTTTCAGCAAAGGAAGTTCTTAGTGCTAGAAAGCAAAAAGCACAGGGGATGCCAGAAAGTGGTG
  TGGCACTTGCTGAAGGATTTCATGCAAGATTACAGGAATTTCAGAATTTTGAACAAATCTTTGAGGAGTG
  TATCTCGCAGTCAGCAGTGAAAACAAAGTTCGAACAGCACACTATCAGAGCTAAACAGATACTAGCTACT
  GTGAAAAACATAATGGATTCAGTAAACCTGGCAGCTGAAGATAAAAGGCATTATTCAGTGGAAGAGAGGG
  AAGACCAAATTGATAGACTGGACTTTATTCGAAACCAGATGAACCTTTTAACACTGGATGTTAAGAAAAA
  AATCAAGGAGGTTACCGAGGAGGTGGCAAACAAAGTTTCATGTGCAATGACAGATGAAATTTGTCGACTG
  TCTGTTTTGGTTGATGAATTTTGTTCAGAGTTTCATCCTAATCCAGATGTATTAAAAATATATAAAAGTG
  AATTAAATAAGCACATAGAGGATGGTATGGGAAGAAATTTGGCTGATCGATGCACCGATGAAGTAAACGC
  CTTAGTGCTTCAGACCCAGCAAGAAATTATTGAAAATTTGAAGCCATTACTTCCAGCTGGTATACAGGAT
  AAACTACATACACTGATCCCTTGCAAGAAATTTGATCTCAGTTATAATCTAAATTACCACAAGTTATGTT
  CAGATTTTCAAGAGGATATTGTATTTCGTTTTTCCCTGGGCTGGTCTTCCCTTGTACATCGATTTTTGGG
  CCCTAGAAATGCTCAAAGGGTGCTCCTAGGATTATCAGAGCCTATCTTTCAGCTCCCTAGATCTTTAGCT
  TCTACTCCCACTGCTCCTACCACTCCAGCAACGCCAGATAATGCATCACAGGAAGAACTCATGATTACAT
  TAGTAACAGGATTGGCGTCCGTTACATCTAGAACTTCTATGGGCATCATTATTGTTGGAGGAGTGATTTG
  GAAAACTATAGGCTGGAAACTCCTATCTGTTTCATTAACTATGTATGGAGCTTTGTATCTTTATGAAAGA
  CTGAGCTGGACCACCCATGCCAAGGAGCGAGCCTTTAAACAGCAGTTTGTAAACTATGCAACTGAAAAAC
  TGAGGATGATTGTTAGCTCCACGAGTGCAAACTGCAGTCACCAAGTAAAACAACAAATAGCTACCACTTT
  TGCTCGCCTGTGCCAACAAGTTGATATTACTCAAAAACAGCTGGAAGAAGAAATTGCTAGATTACCCAAA
  GAAATAGATCAGTTGGAGAAAATACAAAACAATTCAAAGCTCTTAAGAAATAAAGCTGTTCAACTTGAAA
  ATGAGCTGGAGAATTTTACTAAGCAGTTTCTACCTTCAAGCAATGAAGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207184 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEPVSPLKHFVLAKKAITAIFDQLLEFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEV
  LSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVN
  QLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSES
  TLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRI
  FFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILAT
  VKNIMDSVNLAAEDKRHYSVEEREDQIDRLDFIRNQMNLLTLDVKKKIKEVTEEVANKVSCAMTDEICRL
  SVLVDEFCSEFHPNPDVLKIYKSELNKHIEDGMGRNLADRCTDEVNALVLQTQQEIIENLKPLLPAGIQD
  KLHTLIPCKKFDLSYNLNYHKLCSDFQEDIVFRFSLGWSSLVHRFLGPRNAQRVLLGLSEPIFQLPRSLA
  STPTAPTTPATPDNASQEELMITLVTGLASVTSRTSMGIIIVGGVIWKTIGWKLLSVSLTMYGALYLYER
  LSWTTHAKERAFKQQFVNYATEKLRMIVSSTSANCSHQVKQQIATTFARLCQQVDITQKQLEEEIARLPK
  EIDQLEKIQNNSKLLRNKAVQLENELENFTKQFLPSSNEES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033540
• ORF Size: 2223 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033540.3
• RefSeq Size: 3527 bp
• RefSeq ORF: 2226 bp
• Locus ID: 55669
• UniProt ID: Q8IWA4
• Cytogenetics: 3q26.33
• Domains: fzo_mitofusin
• MW: 84.2 kDa
• Gene Summary: The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]