Protocadherin beta 12 (PCDHB12) (NM_018932) Human Tagged ORF Clone
$728.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Protocadherin beta 12 |
| Synonyms | PCDH-BETA12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_018932 |
| ORF Size | 2385 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018932.4 |
| RefSeq Size | 3422 bp |
| RefSeq ORF | 2388 bp |
| Locus ID | 56124 |
| UniProt ID | Q9Y5F1 |
| Cytogenetics | 5q31.3 |
| Protein Families | Transmembrane |
| MW | 86.8 kDa |
| Summary | This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008] |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018932" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC207172L3 | Lenti ORF clone of Human protocadherin beta 12 (PCDHB12), Myc-DDK-tagged | 10 ug |
$1,028.00
|
|
| RC207172L4 | Lenti ORF clone of Human protocadherin beta 12 (PCDHB12), mGFP tagged | 10 ug |
$1,028.00
|
|
| RG207172 | PCDHB12 (tGFP-tagged) - Human protocadherin beta 12 (PCDHB12) | 10 ug |
$928.00
|
|
| SC113330 | PCDHB12 (untagged)-Human protocadherin beta 12 (PCDHB12) | 10 ug |
$729.00
|
|
Plasmid Map for RC207172
Download sequence for
Cloning scheme for RC207172
Circular map for RC207172
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
- Price:
- Actual Price: